Human anticentromere antibodies

Distribution, characterization of antigens, and effect on microtubule organization

John Cox, Eric A. Schenk, J. B. Olmsted

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Properties of human anticentromere autoantibodies were analyzed. In intact cells or isolated cell fractions, these sera stain the centromeres of mitotic chromosomes and discrete speckles (prekinetochores) in nuclei. Staining is also retained in matrix preparations from nuclei or chromosomes. Immunoprecipitation or immunoblotting demonstrates protein antigens of 14, 20, 23, and 34 kd in HeLa nuclei and chromosomes; immunoprecipitates of nuclei also contain a protein of 15.5 kd. Matrix preparations contain only the 20, 23, and 34 kd species. Absorption of the anticentromere serum with any one of the four nuclear antigens immobilized on nitrocellulose is sufficient to eliminate centromere staining. Using a lysed cell model for microtubule nucleation, anticentromere sera are shown to inhibit specifically the organization of microtubules at the kinetochore.

Original languageEnglish (US)
Pages (from-to)331-339
Number of pages9
JournalCell
Volume35
Issue number1
DOIs
StatePublished - Jan 1 1983

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Chromosomes
Microtubules
Centromere
Antigens
Serum
Staining and Labeling
Kinetochores
Nuclear Antigens
Collodion
Speckle
Immunoprecipitation
Immunoblotting
Autoantibodies
Proteins
Nucleation
Coloring Agents
anticentromere antibody

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Human anticentromere antibodies : Distribution, characterization of antigens, and effect on microtubule organization. / Cox, John; Schenk, Eric A.; Olmsted, J. B.

In: Cell, Vol. 35, No. 1, 01.01.1983, p. 331-339.

Research output: Contribution to journalArticle

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