Human cholesterol side-chain cleavage enzyme, P450scc

cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta

B. C. Chung, Karla Matteson, R. Voutilainen, T. K. Mohandas, W. L. Miller

Research output: Contribution to journalArticle

294 Citations (Scopus)

Abstract

Conversion of cholesterol to pregnenolone is mediated by P450scc [cholesterol, reduced-adrenal-ferrodoxin: oxygen oxidoreductase (side-chain-cleaving), EC 1.14.15.67]. RNA from several human adrenal samples was translated in vitro and immunoprecipitated with anti-bovine P450scc, indicating that P450scc mRNA represents about 0.5% of human adrenal mRNA in normal, hypertrophied, and malignant adrenals. A 1626-base-pair human adrenal P450scc cDNA was cloned in bacteriophage λgt10. Primer extension data indicated P450scc mRNA is about 1850 bases long and that all adrenal P450scc mRNA has the same 5' end. A full-length clone containing 1821 bases was obtained from a human testis cDNA library to yield the complete sequence. The encoded human preP450scc contains 521 amino acids with a molecular weight of 60189.65. The testis and adrenal sequences were identical; the human cDNA and amino acid sequences are 82% and 72% homologous, respectively, with the bovine sequences. P450scc cDNA was used to probe DNA from a panel of mouse-human somatic cell hybrids, showing that the single human P450scc gene lies on chromosome 15. The human P450scc gene is expressed in the placenta in early and midgestation; primary cultures of placental tissue indicate P450scc mRNA accumulates in response to cyclic AMP.

Original languageEnglish (US)
Pages (from-to)8962-8966
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number23
DOIs
StatePublished - Jan 1 1986

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Cholesterol Side-Chain Cleavage Enzyme
Chromosomes, Human, Pair 15
Placenta
Organism Cloning
Complementary DNA
Genes
Messenger RNA
Testis
Cholesterol
Pregnenolone
Hybrid Cells
DNA Probes
Gene Library
Base Pairing
Cyclic AMP
Bacteriophages
Amino Acid Sequence
Oxidoreductases
Clone Cells
Molecular Weight

All Science Journal Classification (ASJC) codes

  • General

Cite this

Human cholesterol side-chain cleavage enzyme, P450scc : cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta. / Chung, B. C.; Matteson, Karla; Voutilainen, R.; Mohandas, T. K.; Miller, W. L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 23, 01.01.1986, p. 8962-8966.

Research output: Contribution to journalArticle

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