Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

Dong Li, Xiao Ying Zou, Ikbale El-Ayachi, Luis O. Romero, Zongdong Yu, Alejandro Iglesias-Linares, Julio Cordero-Morales, George Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The potential of human mesenchymal stromal/stem cells (MSCs) including oral stem cells (OSCs) as a cell source to derive functional neurons has been inconclusive. Here we tested a number of human OSCs for their neurogenic potential compared to non-OSCs and employed various neurogenic induction methods. OSCs including dental pulp stem cells (DPSCs), gingiva-derived mesenchymal stem cells (GMSCs), stem cells from apical papilla and non-OSCs including bone marrow MSCs (BMMSCs), foreskin fibroblasts and dermal fibroblasts using non-neurosphere-mediated or neurosphere-mediated methods to guide them toward neuronal lineages. Cells were subjected to RT-qPCR, immunocytofluorescence to detect the expression of neurogenic genes or electrophysiological analysis at final stage of maturation. We found that induced DPSCs and GMSCs overall appeared to be more neurogenic compared to other cells either morphologically or levels of neurogenic gene expression. Nonetheless, of all the neural induction methods employed, only one neurosphere-mediated method yielded electrophysiological properties of functional neurons. Under this method, cells expressed increased neural stem cell markers, nestin and SOX1, in the first phase of differentiation. Neuronal-like cells expressed βIII-tubulin, CNPase, GFAP, MAP-2, NFM, pan-Nav, GAD67, Nav1.6, NF1, NSE, PSD95, and synapsin after the second phase of differentiation to maturity. Electrophysiological experiments revealed that 8.3% of DPSC-derived neuronal cells and 21.2% of GMSC-derived neuronal cells displayed action potential, although no spontaneous excitatory/inhibitory postsynaptic action potential was observed. We conclude that DPSCs and GMSCs have the potential to become neuronal cells in vitro, therefore, these cells may be used as a source for neural regeneration.

Original languageEnglish (US)
Pages (from-to)67-81
Number of pages15
JournalStem Cell Reviews and Reports
Volume15
Issue number1
DOIs
StatePublished - Feb 15 2019

Fingerprint

Dental Pulp
Mesenchymal Stromal Cells
Action Potentials
Stem Cells
Gingiva
2',3'-Cyclic-Nucleotide Phosphodiesterases
Fibroblasts
In Vitro Techniques
Synapsins
Foreskin
Gene Expression
Neurons
Nestin
Inhibitory Postsynaptic Potentials
Neural Stem Cells
Tubulin
Regeneration

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Cancer Research

Cite this

Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation. / Li, Dong; Zou, Xiao Ying; El-Ayachi, Ikbale; Romero, Luis O.; Yu, Zongdong; Iglesias-Linares, Alejandro; Cordero-Morales, Julio; Huang, George.

In: Stem Cell Reviews and Reports, Vol. 15, No. 1, 15.02.2019, p. 67-81.

Research output: Contribution to journalArticle

Li, Dong ; Zou, Xiao Ying ; El-Ayachi, Ikbale ; Romero, Luis O. ; Yu, Zongdong ; Iglesias-Linares, Alejandro ; Cordero-Morales, Julio ; Huang, George. / Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation. In: Stem Cell Reviews and Reports. 2019 ; Vol. 15, No. 1. pp. 67-81.
@article{e2ff803adfe14a52a950e650a4f67360,
title = "Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation",
abstract = "The potential of human mesenchymal stromal/stem cells (MSCs) including oral stem cells (OSCs) as a cell source to derive functional neurons has been inconclusive. Here we tested a number of human OSCs for their neurogenic potential compared to non-OSCs and employed various neurogenic induction methods. OSCs including dental pulp stem cells (DPSCs), gingiva-derived mesenchymal stem cells (GMSCs), stem cells from apical papilla and non-OSCs including bone marrow MSCs (BMMSCs), foreskin fibroblasts and dermal fibroblasts using non-neurosphere-mediated or neurosphere-mediated methods to guide them toward neuronal lineages. Cells were subjected to RT-qPCR, immunocytofluorescence to detect the expression of neurogenic genes or electrophysiological analysis at final stage of maturation. We found that induced DPSCs and GMSCs overall appeared to be more neurogenic compared to other cells either morphologically or levels of neurogenic gene expression. Nonetheless, of all the neural induction methods employed, only one neurosphere-mediated method yielded electrophysiological properties of functional neurons. Under this method, cells expressed increased neural stem cell markers, nestin and SOX1, in the first phase of differentiation. Neuronal-like cells expressed βIII-tubulin, CNPase, GFAP, MAP-2, NFM, pan-Nav, GAD67, Nav1.6, NF1, NSE, PSD95, and synapsin after the second phase of differentiation to maturity. Electrophysiological experiments revealed that 8.3{\%} of DPSC-derived neuronal cells and 21.2{\%} of GMSC-derived neuronal cells displayed action potential, although no spontaneous excitatory/inhibitory postsynaptic action potential was observed. We conclude that DPSCs and GMSCs have the potential to become neuronal cells in vitro, therefore, these cells may be used as a source for neural regeneration.",
author = "Dong Li and Zou, {Xiao Ying} and Ikbale El-Ayachi and Romero, {Luis O.} and Zongdong Yu and Alejandro Iglesias-Linares and Julio Cordero-Morales and George Huang",
year = "2019",
month = "2",
day = "15",
doi = "10.1007/s12015-018-9854-5",
language = "English (US)",
volume = "15",
pages = "67--81",
journal = "Stem Cell Reviews",
issn = "1550-8943",
publisher = "Humana Press",
number = "1",

}

TY - JOUR

T1 - Human Dental Pulp Stem Cells and Gingival Mesenchymal Stem Cells Display Action Potential Capacity In Vitro after Neuronogenic Differentiation

AU - Li, Dong

AU - Zou, Xiao Ying

AU - El-Ayachi, Ikbale

AU - Romero, Luis O.

AU - Yu, Zongdong

AU - Iglesias-Linares, Alejandro

AU - Cordero-Morales, Julio

AU - Huang, George

PY - 2019/2/15

Y1 - 2019/2/15

N2 - The potential of human mesenchymal stromal/stem cells (MSCs) including oral stem cells (OSCs) as a cell source to derive functional neurons has been inconclusive. Here we tested a number of human OSCs for their neurogenic potential compared to non-OSCs and employed various neurogenic induction methods. OSCs including dental pulp stem cells (DPSCs), gingiva-derived mesenchymal stem cells (GMSCs), stem cells from apical papilla and non-OSCs including bone marrow MSCs (BMMSCs), foreskin fibroblasts and dermal fibroblasts using non-neurosphere-mediated or neurosphere-mediated methods to guide them toward neuronal lineages. Cells were subjected to RT-qPCR, immunocytofluorescence to detect the expression of neurogenic genes or electrophysiological analysis at final stage of maturation. We found that induced DPSCs and GMSCs overall appeared to be more neurogenic compared to other cells either morphologically or levels of neurogenic gene expression. Nonetheless, of all the neural induction methods employed, only one neurosphere-mediated method yielded electrophysiological properties of functional neurons. Under this method, cells expressed increased neural stem cell markers, nestin and SOX1, in the first phase of differentiation. Neuronal-like cells expressed βIII-tubulin, CNPase, GFAP, MAP-2, NFM, pan-Nav, GAD67, Nav1.6, NF1, NSE, PSD95, and synapsin after the second phase of differentiation to maturity. Electrophysiological experiments revealed that 8.3% of DPSC-derived neuronal cells and 21.2% of GMSC-derived neuronal cells displayed action potential, although no spontaneous excitatory/inhibitory postsynaptic action potential was observed. We conclude that DPSCs and GMSCs have the potential to become neuronal cells in vitro, therefore, these cells may be used as a source for neural regeneration.

AB - The potential of human mesenchymal stromal/stem cells (MSCs) including oral stem cells (OSCs) as a cell source to derive functional neurons has been inconclusive. Here we tested a number of human OSCs for their neurogenic potential compared to non-OSCs and employed various neurogenic induction methods. OSCs including dental pulp stem cells (DPSCs), gingiva-derived mesenchymal stem cells (GMSCs), stem cells from apical papilla and non-OSCs including bone marrow MSCs (BMMSCs), foreskin fibroblasts and dermal fibroblasts using non-neurosphere-mediated or neurosphere-mediated methods to guide them toward neuronal lineages. Cells were subjected to RT-qPCR, immunocytofluorescence to detect the expression of neurogenic genes or electrophysiological analysis at final stage of maturation. We found that induced DPSCs and GMSCs overall appeared to be more neurogenic compared to other cells either morphologically or levels of neurogenic gene expression. Nonetheless, of all the neural induction methods employed, only one neurosphere-mediated method yielded electrophysiological properties of functional neurons. Under this method, cells expressed increased neural stem cell markers, nestin and SOX1, in the first phase of differentiation. Neuronal-like cells expressed βIII-tubulin, CNPase, GFAP, MAP-2, NFM, pan-Nav, GAD67, Nav1.6, NF1, NSE, PSD95, and synapsin after the second phase of differentiation to maturity. Electrophysiological experiments revealed that 8.3% of DPSC-derived neuronal cells and 21.2% of GMSC-derived neuronal cells displayed action potential, although no spontaneous excitatory/inhibitory postsynaptic action potential was observed. We conclude that DPSCs and GMSCs have the potential to become neuronal cells in vitro, therefore, these cells may be used as a source for neural regeneration.

UR - http://www.scopus.com/inward/record.url?scp=85055557589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85055557589&partnerID=8YFLogxK

U2 - 10.1007/s12015-018-9854-5

DO - 10.1007/s12015-018-9854-5

M3 - Article

VL - 15

SP - 67

EP - 81

JO - Stem Cell Reviews

JF - Stem Cell Reviews

SN - 1550-8943

IS - 1

ER -