Human herpes virus 6 in archival cardiac tissues from children with idiopathic dilated cardiomyopathy or congenital heart disease

M. Comar, P. D'Agaro, C. Campello, A. Poli, J. P. Breinholt, Jeffrey Towbin, M. Vatta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: To explore the possible role of human herpes virus 6 (HHV-6) in cardiac disorders in childhood in a retrospective study on archival specimens of explanted hearts. Methods: 16 children (median age at transplantation 11.0 years) with idiopathic dilated cardiomyopathy (DCM) and 19 children (median age at transplantation 1.0 year) with congenital heart disease (CHD), previously found to be negative for other cardiotropic viruses such as enteroviruses, adenovirus, parvovirus B19, cytomegalovirus and Epstein-Barr virus, were tested for HHV-6 by quantitative real-time PCR and by genotyping. In addition, HHV-7/8 infection was investigated by qualitative PCR. Results: HHV-6 B variant was detected in 11 of 35 samples (31.4%) with a mean viral load of 3.1 × 102 copies/u.g of DNA. When assessed by heart disorder, the prevalence was different in the two groups (43.7% in DCM and 21% in CHD) while the mean viral loads were similar. In a logistic multivariate analysis HHV-6 was independently associated with DCM, taking CHD as reference and adjusting for age (best estimate: OR = 6.94; 95% CI 1.00 to 49.85; p = 0.05). Conclusions: Although the clinical significance of the results is unknown, HHV-6 B genome is frequently detected in explanted hearts from children with DCM and to a lesser extent with CHD, thus adding evidence for HHV-6 cardiac involvement.

Original languageEnglish (US)
Pages (from-to)80-83
Number of pages4
JournalJournal of Clinical Pathology
Volume62
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Dilated Cardiomyopathy
Heart Diseases
Viruses
Cercopithecine Herpesvirus 1
Viral Load
Human Herpesvirus 7
Transplantation
Human Herpesvirus 8
Parvovirus
Enterovirus
Cytomegalovirus
Human Herpesvirus 4
Adenoviridae
Real-Time Polymerase Chain Reaction
Multivariate Analysis
Retrospective Studies
Genome
Polymerase Chain Reaction
DNA
Infection

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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Human herpes virus 6 in archival cardiac tissues from children with idiopathic dilated cardiomyopathy or congenital heart disease. / Comar, M.; D'Agaro, P.; Campello, C.; Poli, A.; Breinholt, J. P.; Towbin, Jeffrey; Vatta, M.

In: Journal of Clinical Pathology, Vol. 62, No. 1, 01.01.2009, p. 80-83.

Research output: Contribution to journalArticle

Comar, M. ; D'Agaro, P. ; Campello, C. ; Poli, A. ; Breinholt, J. P. ; Towbin, Jeffrey ; Vatta, M. / Human herpes virus 6 in archival cardiac tissues from children with idiopathic dilated cardiomyopathy or congenital heart disease. In: Journal of Clinical Pathology. 2009 ; Vol. 62, No. 1. pp. 80-83.
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abstract = "Objective: To explore the possible role of human herpes virus 6 (HHV-6) in cardiac disorders in childhood in a retrospective study on archival specimens of explanted hearts. Methods: 16 children (median age at transplantation 11.0 years) with idiopathic dilated cardiomyopathy (DCM) and 19 children (median age at transplantation 1.0 year) with congenital heart disease (CHD), previously found to be negative for other cardiotropic viruses such as enteroviruses, adenovirus, parvovirus B19, cytomegalovirus and Epstein-Barr virus, were tested for HHV-6 by quantitative real-time PCR and by genotyping. In addition, HHV-7/8 infection was investigated by qualitative PCR. Results: HHV-6 B variant was detected in 11 of 35 samples (31.4{\%}) with a mean viral load of 3.1 × 102 copies/u.g of DNA. When assessed by heart disorder, the prevalence was different in the two groups (43.7{\%} in DCM and 21{\%} in CHD) while the mean viral loads were similar. In a logistic multivariate analysis HHV-6 was independently associated with DCM, taking CHD as reference and adjusting for age (best estimate: OR = 6.94; 95{\%} CI 1.00 to 49.85; p = 0.05). Conclusions: Although the clinical significance of the results is unknown, HHV-6 B genome is frequently detected in explanted hearts from children with DCM and to a lesser extent with CHD, thus adding evidence for HHV-6 cardiac involvement.",
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N2 - Objective: To explore the possible role of human herpes virus 6 (HHV-6) in cardiac disorders in childhood in a retrospective study on archival specimens of explanted hearts. Methods: 16 children (median age at transplantation 11.0 years) with idiopathic dilated cardiomyopathy (DCM) and 19 children (median age at transplantation 1.0 year) with congenital heart disease (CHD), previously found to be negative for other cardiotropic viruses such as enteroviruses, adenovirus, parvovirus B19, cytomegalovirus and Epstein-Barr virus, were tested for HHV-6 by quantitative real-time PCR and by genotyping. In addition, HHV-7/8 infection was investigated by qualitative PCR. Results: HHV-6 B variant was detected in 11 of 35 samples (31.4%) with a mean viral load of 3.1 × 102 copies/u.g of DNA. When assessed by heart disorder, the prevalence was different in the two groups (43.7% in DCM and 21% in CHD) while the mean viral loads were similar. In a logistic multivariate analysis HHV-6 was independently associated with DCM, taking CHD as reference and adjusting for age (best estimate: OR = 6.94; 95% CI 1.00 to 49.85; p = 0.05). Conclusions: Although the clinical significance of the results is unknown, HHV-6 B genome is frequently detected in explanted hearts from children with DCM and to a lesser extent with CHD, thus adding evidence for HHV-6 cardiac involvement.

AB - Objective: To explore the possible role of human herpes virus 6 (HHV-6) in cardiac disorders in childhood in a retrospective study on archival specimens of explanted hearts. Methods: 16 children (median age at transplantation 11.0 years) with idiopathic dilated cardiomyopathy (DCM) and 19 children (median age at transplantation 1.0 year) with congenital heart disease (CHD), previously found to be negative for other cardiotropic viruses such as enteroviruses, adenovirus, parvovirus B19, cytomegalovirus and Epstein-Barr virus, were tested for HHV-6 by quantitative real-time PCR and by genotyping. In addition, HHV-7/8 infection was investigated by qualitative PCR. Results: HHV-6 B variant was detected in 11 of 35 samples (31.4%) with a mean viral load of 3.1 × 102 copies/u.g of DNA. When assessed by heart disorder, the prevalence was different in the two groups (43.7% in DCM and 21% in CHD) while the mean viral loads were similar. In a logistic multivariate analysis HHV-6 was independently associated with DCM, taking CHD as reference and adjusting for age (best estimate: OR = 6.94; 95% CI 1.00 to 49.85; p = 0.05). Conclusions: Although the clinical significance of the results is unknown, HHV-6 B genome is frequently detected in explanted hearts from children with DCM and to a lesser extent with CHD, thus adding evidence for HHV-6 cardiac involvement.

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