Human herpes virus 8 in skin disease

An immunoperoxidase analysis

Robert Page, Michael Harvey, Roy King, Paul Googe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human herpes virus 8 (HHV8) has been consistently implicated in the pathogenesis of Kaposi's sarcoma (KS) and has been studied in a variety of skin lesions, principally by means of polymerase chain reaction. With this in mind, we sought to characterize a variety of skin lesions by means of paraffin immunoperoxidase studies using the antibody to the HHV8 latent nuclear antigen 1. We studied a variety of benign and malignant vascular lesions, including KS (eight cases), hemangiomas (six), angiolymphoid hyperplasia (three), and angiosarcoma (two). In addition, we assessed antibody staining in lymphomatoid papulosis (LYP; 5 cases), pityriasis lichenoides et varioliformis acuta (5), and pityriasis rosea (5) as well as a variety of mesenchymal and epithelial primary cutaneous tumors (nine cases). In accordance with previous studies, all cases of KS (8/8) showed positive staining with HHV8 in a granular nuclear pattern; staining was limited to tumor cells. None of the other cutaneous tumors or inflammatory conditions showed any staining (0/35). HHV8 is a useful tool in confirming the diagnosis of cutaneous KS. Consistent with previous studies, HHV8 was not demonstrated in LYP and, novel to this study, was not found in pityriasis rosea or pityriasis lichenoides et varioliformis acuta. Paraffin immunoperoxidase with HHV8 latent nuclear antigen 1 is a viable alternative to polymerase chain reaction and can be used in diagnostic and research capacities with consistent results.

Original languageEnglish (US)
Pages (from-to)67-70
Number of pages4
JournalJournal of Histotechnology
Volume28
Issue number2
DOIs
StatePublished - Jan 1 2005

Fingerprint

Skin Diseases
Kaposi's Sarcoma
Viruses
Pityriasis Lichenoides
Pityriasis Rosea
Skin
Staining and Labeling
Paraffin
Lymphomatoid Papulosis
Neoplasms
Polymerase Chain Reaction
Hemangiosarcoma
Antibodies
Hemangioma
Hyperplasia
Blood Vessels
Research

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology
  • Medical Laboratory Technology

Cite this

Human herpes virus 8 in skin disease : An immunoperoxidase analysis. / Page, Robert; Harvey, Michael; King, Roy; Googe, Paul.

In: Journal of Histotechnology, Vol. 28, No. 2, 01.01.2005, p. 67-70.

Research output: Contribution to journalArticle

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