Human immunoglobulin kappa light chain genes of subgroups II and III

H. Gustav Klobeck, Alfons Meindl, Gabriele Combriato, Alan Solomon, Hans G. Zachau

Research output: Contribution to journalArticle

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Abstract

The first complete sequences of functionally rearranged VK genes (abbreviations ref.l) of subgroups II and III are reported. The genes have been cloned from lyraphoid cell lines synthesizing KII or KIII light chains as evidenced from immunochemical analyses with anti-VK subgroup-specific antisera. These data, together with the sequence of a KIV gene (described in the accompanying paper) and those of previously published KI genes make possible a comparison of genes representative of the four known V region subgroups of human K light chains. The VKII gene is distinguished from the VKI, VKIII, and VKIV genes by a much longer intron within the leader sequence: 426 bp vs ca. 120-220 bp. Blot hybridization experiments with human DNA digests using probes from the KII and KIII genes and from the respective upstream regions help to define subgroup specific probes and hybridization conditions.

Original languageEnglish (US)
Pages (from-to)6499-6513
Number of pages15
JournalNucleic acids research
Volume13
Issue number18
DOIs
StatePublished - Sep 25 1985

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Immunoglobulin Light Chain Genes
Genes
Light
Introns
Immune Sera
Cell Line

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Human immunoglobulin kappa light chain genes of subgroups II and III. / Klobeck, H. Gustav; Meindl, Alfons; Combriato, Gabriele; Solomon, Alan; Zachau, Hans G.

In: Nucleic acids research, Vol. 13, No. 18, 25.09.1985, p. 6499-6513.

Research output: Contribution to journalArticle

Klobeck, HG, Meindl, A, Combriato, G, Solomon, A & Zachau, HG 1985, 'Human immunoglobulin kappa light chain genes of subgroups II and III', Nucleic acids research, vol. 13, no. 18, pp. 6499-6513. https://doi.org/10.1093/nar/13.18.6499
Klobeck, H. Gustav ; Meindl, Alfons ; Combriato, Gabriele ; Solomon, Alan ; Zachau, Hans G. / Human immunoglobulin kappa light chain genes of subgroups II and III. In: Nucleic acids research. 1985 ; Vol. 13, No. 18. pp. 6499-6513.
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