Human leukocyte elastase inhibition by Bowman-Birk soybean inhibitor. Discrimination of the inhibition mechanisms

Natalia I. Larionova, Inna Gladysheva, Dimitri P. Gladyshev

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The reaction between human leukocyte elastase and soybean Bowman-Birk inhibitor has been studied. The inhibition was found to be due to slow tight binding of the inhibitor. The interaction of BBI with HLE was shown to involve two steps: the rapid formation of an initial EI complex, with a K(i) of 28 nM, followed by a slow equilibrium conversion to a tighter-binding EI* complex with a final K(i)* of 2.3 nM. At pH 7.5 and 25°C, k(on) was 3.5 x 104 M-1 s-1 and k(off) was 1.0 x 10-4 s-1.

Original languageEnglish (US)
Pages (from-to)245-248
Number of pages4
JournalFEBS Letters
Volume404
Issue number2-3
DOIs
StatePublished - Mar 10 1997

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Leukocyte Elastase
Soybeans

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Human leukocyte elastase inhibition by Bowman-Birk soybean inhibitor. Discrimination of the inhibition mechanisms. / Larionova, Natalia I.; Gladysheva, Inna; Gladyshev, Dimitri P.

In: FEBS Letters, Vol. 404, No. 2-3, 10.03.1997, p. 245-248.

Research output: Contribution to journalArticle

Larionova, Natalia I. ; Gladysheva, Inna ; Gladyshev, Dimitri P. / Human leukocyte elastase inhibition by Bowman-Birk soybean inhibitor. Discrimination of the inhibition mechanisms. In: FEBS Letters. 1997 ; Vol. 404, No. 2-3. pp. 245-248.
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