Human sperm-specific peptide vaccine that causes long-term reversible contraception

Rajesh K. Naz, Subhash Chauhan

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A novel dodecamer peptide sequence, YLP12, was identified on human sperm that is involved in oocyte binding. We investigated its immunocontraceptive effects in a murine model. A vaccine was prepared by conjugating the synthetic YLP12 peptide with the binding subunit of recombinant cholera toxin. Vaccination of female mice by i.m. or intranasal routes without any additional adjuvant induced a sperm-specific immune response in serum and the vaginal tract that caused a long-term contraceptive state. Fertility was fully regained when antibody reactivity diminished at 305-322 days. The contraceptive effect was also completely reversed voluntarily by intravaginal administration of the peptide. Antibodies affected fertility at the prefertilization stage by inhibiting sperm capacitation and the acrosome reaction, and sperm-oocyte binding. The peptide sequence is an epitope of a 50 ± 5-kDa membrane protein localized on the acrosome and tail of spermatozoa. Thus, the sperm-specific YLP12 is an attractive candidate for contraceptive vaccine.

Original languageEnglish (US)
Pages (from-to)674-680
Number of pages7
JournalBiology of Reproduction
Volume67
Issue number2
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

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Subunit Vaccines
Contraception
Spermatozoa
Peptides
Contraceptive Agents
Oocytes
Fertility
Contraceptive Vaccines
Intravaginal Administration
Sperm Capacitation
Acrosome Reaction
Acrosome
Antibodies
Cholera Toxin
Tail
Epitopes
Membrane Proteins
Vaccination
Vaccines
Serum

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Human sperm-specific peptide vaccine that causes long-term reversible contraception. / Naz, Rajesh K.; Chauhan, Subhash.

In: Biology of Reproduction, Vol. 67, No. 2, 01.01.2002, p. 674-680.

Research output: Contribution to journalArticle

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