Humoral and cellular sensitivity to collagen in type II collagen induced arthritis in rats

D. E. Trentham, A. S. Townes, Andrew Kang, J. R. David

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

The authors have recently described a new animal model of arthritis induced by intradermal injection of a distinct type of collagen found in cartilage (type II collagen). Since immunologic sensivity to collagen could play a role in the pathogenesis of this type II collagen-induced arthritis in rats, the ability of purified types of native collagens to induce cellular and humoral responses was quantified by antigen-induced tritiated thymidine incorporation into lymphocytes by collagen and passive hemagglutination, respectively. Rats injected intradermally with native heterologous or homologous type II collagens in adjuvant developed type-specific cellular as well as humoral reactivity. Types I and III collagens were less immunogenic than was type II. The latter collagen induced brisk cellular and humoral responses that were equivalent whether complete Freund's adjuvant or incomplete Freund's adjuvant were employed. Both responses could be induced by native type II collagens modified by limited pepsin digestion, indicating that they are not attributable to determinants in the telopeptide regions of the molecule. Thus, these studies demonstrate the unique immunogenic as well as arthitogenic properties of the type II collagen molecule and indicate that both result from a helical conformation of its structurally distinct α-chains. Further, they suggest that type II collagen may, by humoral or cellular mechanisms, provoke or perpetuate inflammation in other arthritic diseases.

Original languageEnglish (US)
Pages (from-to)89-96
Number of pages8
JournalJournal of Clinical Investigation
Volume61
Issue number1
DOIs
StatePublished - Jan 1 1978

Fingerprint

Experimental Arthritis
Collagen Type II
Collagen
Arthritis
Intradermal Injections
Collagen Type III
Freund's Adjuvant
Pepsin A
Hemagglutination
Collagen Type I
Thymidine
Digestion
Animal Models
Lymphocytes
Inflammation
Antigens

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Humoral and cellular sensitivity to collagen in type II collagen induced arthritis in rats. / Trentham, D. E.; Townes, A. S.; Kang, Andrew; David, J. R.

In: Journal of Clinical Investigation, Vol. 61, No. 1, 01.01.1978, p. 89-96.

Research output: Contribution to journalArticle

@article{faf36bd508c74b7c94c1f326a8c3c9f6,
title = "Humoral and cellular sensitivity to collagen in type II collagen induced arthritis in rats",
abstract = "The authors have recently described a new animal model of arthritis induced by intradermal injection of a distinct type of collagen found in cartilage (type II collagen). Since immunologic sensivity to collagen could play a role in the pathogenesis of this type II collagen-induced arthritis in rats, the ability of purified types of native collagens to induce cellular and humoral responses was quantified by antigen-induced tritiated thymidine incorporation into lymphocytes by collagen and passive hemagglutination, respectively. Rats injected intradermally with native heterologous or homologous type II collagens in adjuvant developed type-specific cellular as well as humoral reactivity. Types I and III collagens were less immunogenic than was type II. The latter collagen induced brisk cellular and humoral responses that were equivalent whether complete Freund's adjuvant or incomplete Freund's adjuvant were employed. Both responses could be induced by native type II collagens modified by limited pepsin digestion, indicating that they are not attributable to determinants in the telopeptide regions of the molecule. Thus, these studies demonstrate the unique immunogenic as well as arthitogenic properties of the type II collagen molecule and indicate that both result from a helical conformation of its structurally distinct α-chains. Further, they suggest that type II collagen may, by humoral or cellular mechanisms, provoke or perpetuate inflammation in other arthritic diseases.",
author = "Trentham, {D. E.} and Townes, {A. S.} and Andrew Kang and David, {J. R.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1172/JCI108929",
language = "English (US)",
volume = "61",
pages = "89--96",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "1",

}

TY - JOUR

T1 - Humoral and cellular sensitivity to collagen in type II collagen induced arthritis in rats

AU - Trentham, D. E.

AU - Townes, A. S.

AU - Kang, Andrew

AU - David, J. R.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - The authors have recently described a new animal model of arthritis induced by intradermal injection of a distinct type of collagen found in cartilage (type II collagen). Since immunologic sensivity to collagen could play a role in the pathogenesis of this type II collagen-induced arthritis in rats, the ability of purified types of native collagens to induce cellular and humoral responses was quantified by antigen-induced tritiated thymidine incorporation into lymphocytes by collagen and passive hemagglutination, respectively. Rats injected intradermally with native heterologous or homologous type II collagens in adjuvant developed type-specific cellular as well as humoral reactivity. Types I and III collagens were less immunogenic than was type II. The latter collagen induced brisk cellular and humoral responses that were equivalent whether complete Freund's adjuvant or incomplete Freund's adjuvant were employed. Both responses could be induced by native type II collagens modified by limited pepsin digestion, indicating that they are not attributable to determinants in the telopeptide regions of the molecule. Thus, these studies demonstrate the unique immunogenic as well as arthitogenic properties of the type II collagen molecule and indicate that both result from a helical conformation of its structurally distinct α-chains. Further, they suggest that type II collagen may, by humoral or cellular mechanisms, provoke or perpetuate inflammation in other arthritic diseases.

AB - The authors have recently described a new animal model of arthritis induced by intradermal injection of a distinct type of collagen found in cartilage (type II collagen). Since immunologic sensivity to collagen could play a role in the pathogenesis of this type II collagen-induced arthritis in rats, the ability of purified types of native collagens to induce cellular and humoral responses was quantified by antigen-induced tritiated thymidine incorporation into lymphocytes by collagen and passive hemagglutination, respectively. Rats injected intradermally with native heterologous or homologous type II collagens in adjuvant developed type-specific cellular as well as humoral reactivity. Types I and III collagens were less immunogenic than was type II. The latter collagen induced brisk cellular and humoral responses that were equivalent whether complete Freund's adjuvant or incomplete Freund's adjuvant were employed. Both responses could be induced by native type II collagens modified by limited pepsin digestion, indicating that they are not attributable to determinants in the telopeptide regions of the molecule. Thus, these studies demonstrate the unique immunogenic as well as arthitogenic properties of the type II collagen molecule and indicate that both result from a helical conformation of its structurally distinct α-chains. Further, they suggest that type II collagen may, by humoral or cellular mechanisms, provoke or perpetuate inflammation in other arthritic diseases.

UR - http://www.scopus.com/inward/record.url?scp=0017874061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017874061&partnerID=8YFLogxK

U2 - 10.1172/JCI108929

DO - 10.1172/JCI108929

M3 - Article

VL - 61

SP - 89

EP - 96

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 1

ER -