Hyaluronic acid immobilized magnetic nanoparticles for active targeting and imaging of macrophages

Medha Kamat, Kheireddine El-Boubbou, David C. Zhu, Teri Lansdell, Xiaowei Lu, Wei Li, Xuefei Huang

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Imaging and targeted delivery to macrophages are promising new approaches to study and treat a variety of inflammatory diseases such as atherosclerosis. In this manuscript, we have designed and synthesized iron oxide based magnetic nanoparticles bearing hyaluronic acid (HA) on the surface to target activated macrophages. The HA-coated nanoparticles were prepared through a co-precipitation procedure followed by postsynthetic functionalization with HA and fluorescein. The nanoparticles were characterized by transmission electron microscopy, thermogravimetric analysis, elemental analysis, dynamic light scattering, and high-resolution magic angle spinning NMR and were biocompatible with cells and colloidally stable in the presence of serum. The HA immobilized on the nanoparticles retained their specific biological recognition with the HA receptor CD44, which is present on activated macrophages in high-affinity forms. Cell uptake studies demonstrated significant uptake of HA nanoparticles by activated macrophage cell line THP-1, which enabled magnetic resonance imaging of THP-1 cells. The uptake of nanoparticles was found to be both HA and CD44 dependent. Interestingly, Prussian blue staining showed that the magnetite cores of the HA-coated nanoparticles were only transiently present inside the cells, thus reducing the potential concerns of nanotoxicity. Furthermore, fluorescein on the nanoparticle was found to be delivered to the cell nucleus. Therefore, with further development, these HA functionalized magnetic nanoparticles can potentially become a useful carrier system for molecular imaging and targeted drug delivery to activated macrophages.

Original languageEnglish (US)
Pages (from-to)2128-2135
Number of pages8
JournalBioconjugate Chemistry
Volume21
Issue number11
DOIs
StatePublished - Nov 17 2010

Fingerprint

Hyaluronic acid
Macrophages
Hyaluronic Acid
Nanoparticles
Imaging techniques
Fluorescein
Bearings (structural)
Cells
Ferrosoferric Oxide
Molecular imaging
Magic angle spinning
Molecular Imaging
Magnetite
Dynamic light scattering
Magnetic resonance
Coprecipitation
Cell Nucleus
Transmission Electron Microscopy
Iron oxides
Thermogravimetric analysis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

Hyaluronic acid immobilized magnetic nanoparticles for active targeting and imaging of macrophages. / Kamat, Medha; El-Boubbou, Kheireddine; Zhu, David C.; Lansdell, Teri; Lu, Xiaowei; Li, Wei; Huang, Xuefei.

In: Bioconjugate Chemistry, Vol. 21, No. 11, 17.11.2010, p. 2128-2135.

Research output: Contribution to journalArticle

Kamat, M, El-Boubbou, K, Zhu, DC, Lansdell, T, Lu, X, Li, W & Huang, X 2010, 'Hyaluronic acid immobilized magnetic nanoparticles for active targeting and imaging of macrophages', Bioconjugate Chemistry, vol. 21, no. 11, pp. 2128-2135. https://doi.org/10.1021/bc100354m
Kamat, Medha ; El-Boubbou, Kheireddine ; Zhu, David C. ; Lansdell, Teri ; Lu, Xiaowei ; Li, Wei ; Huang, Xuefei. / Hyaluronic acid immobilized magnetic nanoparticles for active targeting and imaging of macrophages. In: Bioconjugate Chemistry. 2010 ; Vol. 21, No. 11. pp. 2128-2135.
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