Hydrogel coated mesh decreases tissue reaction resulting from polypropylene mesh implant

implication in hernia repair

D. P. Poppas, J. J. Sung, C. M. Magro, J. Chen, J. P. Toyohara, Bruce Ramshaw, D. Felsen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: Use of polypropylene mesh (PPM) in hernia repair is associated with tissue reactivity. We examined, in a rat model, a novel non-biodegradable hydrogel coated PPM which may allow for decreased inflammation and a decreased foreign body reaction. Methods: Through a dorsal midline incision, a 2 cm × 2 cm section of PPM (either coated or uncoated) was placed on the fascial surface 1.5 cm from the incision on the dorsal wall of Sprague–Dawley rats. At 2 and 12 weeks after placement, the PPM and surrounding tissue were harvested. A board-certified dermatopathologist examined H&E stained slides for fibrosis and foreign body reaction. In addition, tissues were stained for apoptotic cells, oxidative damage, macrophages, fibroblasts, neovascularization and metalloproteases. Results: At 2 and 12 weeks, there was a greater than 95 % decrease in foreign body giant cells in coated PPM samples compared to uncoated; fibrosis was decreased by 50 %. At 2 and 12 weeks, oxidative damage, fibroblast accumulation, apoptosis and macrophages were significantly decreased in coated PPM samples compared to uncoated PPM. Conclusion: These results demonstrate that a non-biodegradable hydrogel coating of PPM led to significant reduction in foreign body reaction, oxidative stress and apoptosis compared to uncoated PPM in vivo, and suggest that this coating could be clinically useful in hernia repair.

Original languageEnglish (US)
Pages (from-to)623-632
Number of pages10
JournalHernia
Volume20
Issue number4
DOIs
StatePublished - Aug 1 2016

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Polypropylenes
Hydrogel
Herniorrhaphy
Foreign-Body Reaction
Fibrosis
Fibroblasts
Macrophages
Foreign Body Giant Cells
Apoptosis
Metalloproteases
Oxidative Stress
Inflammation

All Science Journal Classification (ASJC) codes

  • Surgery

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Hydrogel coated mesh decreases tissue reaction resulting from polypropylene mesh implant : implication in hernia repair. / Poppas, D. P.; Sung, J. J.; Magro, C. M.; Chen, J.; Toyohara, J. P.; Ramshaw, Bruce; Felsen, D.

In: Hernia, Vol. 20, No. 4, 01.08.2016, p. 623-632.

Research output: Contribution to journalArticle

Poppas, D. P. ; Sung, J. J. ; Magro, C. M. ; Chen, J. ; Toyohara, J. P. ; Ramshaw, Bruce ; Felsen, D. / Hydrogel coated mesh decreases tissue reaction resulting from polypropylene mesh implant : implication in hernia repair. In: Hernia. 2016 ; Vol. 20, No. 4. pp. 623-632.
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