Hydrogen peroxide activation of cytosolic phospholipase A2 in vascular smooth muscle cells

Rao Gadiparthi, M. S. Runge, R. W. Alexander

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

We have reported previously that hydrogen peroxide induces arachidonic acid release from prelabeled vascular smooth muscle cells. Here, we studied the effect of hydrogen peroxide on the phosphorylation of cytosolic phospholipase A2 in these cells. Hydrogen peroxide induced a rapid, time-dependent increase in the phosphorylation of cytosolic phospholipase A2. Hydrogen peroxide also increased arachidonic acid release from prelabeled cells in a time-dependent manner similar to that of phosphorylation of cytosolic phospholipase A2. Protein kinase C depletion significantly inhibited the hydrogen peroxide-stimulated cytosolic phospholipase A2 phosphorylation and arachidonic acid release. Hydrogen peroxide caused a time-dependent increase in mitogen activated protein kinase activity. Taken together, these findings suggest that cytosolic phospholipase A2 may, at least in part, contribute to arachidonic acid release induced by hydrogen peroxide and this effect appears to be mediated by protein kinase C and mitogen activated protein kinase.

Original languageEnglish (US)
Pages (from-to)67-72
Number of pages6
JournalBBA - Molecular Cell Research
Volume1265
Issue number1
DOIs
StatePublished - Feb 16 1995

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Cytosolic Phospholipases A2
Vascular Smooth Muscle
Hydrogen Peroxide
Smooth Muscle Myocytes
Arachidonic Acid
Phosphorylation
Mitogen-Activated Protein Kinases
Protein Kinase C

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Hydrogen peroxide activation of cytosolic phospholipase A2 in vascular smooth muscle cells. / Gadiparthi, Rao; Runge, M. S.; Alexander, R. W.

In: BBA - Molecular Cell Research, Vol. 1265, No. 1, 16.02.1995, p. 67-72.

Research output: Contribution to journalArticle

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