Hydrogen peroxide sensing and cytotoxicity activity of Acacia lignin stabilized silver nanoparticles

Keshaw Ram Aadil, Anand Barapatre, Avtar Meena, Harit Jha

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The study is aimed at detection of hydrogen peroxide (H 2 O 2 ) using Acacia lignin mediated silver nanoparticles (AGNPs). The synthesis of AGNPs was achieved at conditions optimized as, 3 ml of 0.02% lignin and 1 mM silver nitrate incubated for 30 min at 80 °C and pH 9. Initial screening of AGNPs was performed by measuring the surface plasmon resonance peak at 410-430 nm using UV-vis spectrophotometer. Transmission electron microscopy, atomic force microscopy, X-ray diffraction and particle size analysis confirmed the spherical shaped face centered cubic structure and 10-50 nm size of AGNPs. The infrared spectroscopy study further revealed that the active functional groups present in lignin were responsible for the reduction of silver ions (Ag + ) to metallic silver (Ag 0 ). Lignin stabilized silver nanoparticles showed good sensitivity and a linear response over wide concentrations of H 2 O 2 (10 -1 to 10 -6 M). Further, the in vitrocytotoxicity activity of the lignin mediated AGNPs (5-500 μg/ml) demonstrated toxicity effects in MCF-7 and A375 cell lines. Thus, lignin stabilized silver nanoparticles based optical sensor for H 2 O 2 could be potentially applied in the determination of reactive oxygen species and toxic chemicals which further expands the importance of lignin stabilized silver nanoparticles.

Original languageEnglish (US)
Pages (from-to)39-47
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume82
DOIs
StatePublished - Jan 1 2016

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Acacia
Lignin
Cytotoxicity
Silver
Hydrogen peroxide
Nanoparticles
Hydrogen Peroxide
Ultraviolet spectrophotometers
Silver Nitrate
Surface Plasmon Resonance
Atomic Force Microscopy
Poisons
MCF-7 Cells
Optical sensors
Surface plasmon resonance
Transmission Electron Microscopy
Particle Size
X-Ray Diffraction
Particle size analysis
Functional groups

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

Hydrogen peroxide sensing and cytotoxicity activity of Acacia lignin stabilized silver nanoparticles. / Aadil, Keshaw Ram; Barapatre, Anand; Meena, Avtar; Jha, Harit.

In: International Journal of Biological Macromolecules, Vol. 82, 01.01.2016, p. 39-47.

Research output: Contribution to journalArticle

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