Hyperthermic cytotoxicity induced by noninvasvie radiofrequency field exposure after delivery of EGFR family targeted gold nanoparticles

Evan Glazer, Warna D. Kaluarachchi, Steven A. Curley

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Noninvasive radiofrequency (RF) fields heat metal nanoparticles in a concentration dependent fashion. Gold nanoparticles are especially interesting for biomedical applications because they not only heat well, but they have an established biosafety profile. Antibody-targeted gold nanoparticles have been used to induce hyperthermic cytotoxicity when exposed to RF fields. Two carcinoma cells lines, Panc-1 and Hep3B, were individually treated with 100 nM panitumumab and trastuzumab antibody conjugated 10 nm gold nanoparticles and subsequently exposed to an RF field for a total generator power of ∼100 kJ. Two days later, control cells treated with antibody labeled gold nanoparticles, but not exposed to the RF field, maintained an average viability of 92.1%±2.5% for Hep 3B cells and 89.1%±2.1% for Panc-1 cells based on flow cytometry. Panc-1 cells treated the same way with subsequent RF field exposure had viability less than 80% (p ∼ 0.001). Hep3B cells showed a similar decrease in viability after trastuzumab-gold treatment (74.5%±6.9%), but not panitumumab. This demonstrates a new and developing use of antibodies, specifically, against EGFR family targets.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
Pages97-98
Number of pages2
StatePublished - Aug 4 2010
Externally publishedYes
Event1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, United States
Duration: Feb 7 2010Feb 10 2010

Other

Other1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
CountryUnited States
CityHouston, TX
Period2/7/102/10/10

Fingerprint

Cytotoxicity
Gold
Nanoparticles
Antibodies
Hot Temperature
Metal Nanoparticles
Flow cytometry
Metal nanoparticles
Flow Cytometry
Cells
Carcinoma
Cell Line
panitumumab
Trastuzumab

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine(all)

Cite this

Glazer, E., Kaluarachchi, W. D., & Curley, S. A. (2010). Hyperthermic cytotoxicity induced by noninvasvie radiofrequency field exposure after delivery of EGFR family targeted gold nanoparticles. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010 (pp. 97-98)

Hyperthermic cytotoxicity induced by noninvasvie radiofrequency field exposure after delivery of EGFR family targeted gold nanoparticles. / Glazer, Evan; Kaluarachchi, Warna D.; Curley, Steven A.

Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. 2010. p. 97-98.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Glazer, E, Kaluarachchi, WD & Curley, SA 2010, Hyperthermic cytotoxicity induced by noninvasvie radiofrequency field exposure after delivery of EGFR family targeted gold nanoparticles. in Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. pp. 97-98, 1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010, Houston, TX, United States, 2/7/10.
Glazer E, Kaluarachchi WD, Curley SA. Hyperthermic cytotoxicity induced by noninvasvie radiofrequency field exposure after delivery of EGFR family targeted gold nanoparticles. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. 2010. p. 97-98
Glazer, Evan ; Kaluarachchi, Warna D. ; Curley, Steven A. / Hyperthermic cytotoxicity induced by noninvasvie radiofrequency field exposure after delivery of EGFR family targeted gold nanoparticles. Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010. 2010. pp. 97-98
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