Hypocretin-I activates G proteins in arousal-related brainstem nuclei of rat

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The hypocretin (hcrt) ligand-receptor system contributes to the maintenance of wakefulness. Hypocretin receptors are coupled to guanine nucleotide binding (G) proteins and the present study tested the hypothesis that hcrt-I would activate G proteins in rat brainstem nuclei known to regulate arousal states. In vitro [35S]GTPγS autoradiography was performed using coronal brain stem sections from six rats. Hcrt-I (200 nM) significantly increased [35S]GTPγS binding over basal levels in locus coeruleus (24%), dorsal raphe nucleus (12%), pontine reticular nucleus, oral part (28%), and pontine reticular nucleus, caudal part (40%). This is the first study to quantify and localize hcrt-I-induced G protein activation in specific brain stem nuclei. The finding that hcrt-I stimulated [35S]GTPγS binding suggests that some hcrt receptors in pontine brain stem couple to inhibitory G proteins.

Original languageEnglish (US)
Pages (from-to)447-450
Number of pages4
JournalNeuroReport
Volume13
Issue number4
DOIs
StatePublished - Mar 25 2002
Externally publishedYes

Fingerprint

Orexin Receptors
Arousal
GTP-Binding Proteins
Brain Stem
Guanine Nucleotides
Locus Coeruleus
Wakefulness
Autoradiography
Carrier Proteins
Maintenance
Ligands
Orexins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Hypocretin-I activates G proteins in arousal-related brainstem nuclei of rat. / Bernard, Rene; Lydic, Ralph; Baghdoyan, Helen.

In: NeuroReport, Vol. 13, No. 4, 25.03.2002, p. 447-450.

Research output: Contribution to journalArticle

@article{1a2e63821355495fbe1b4b8301af1df1,
title = "Hypocretin-I activates G proteins in arousal-related brainstem nuclei of rat",
abstract = "The hypocretin (hcrt) ligand-receptor system contributes to the maintenance of wakefulness. Hypocretin receptors are coupled to guanine nucleotide binding (G) proteins and the present study tested the hypothesis that hcrt-I would activate G proteins in rat brainstem nuclei known to regulate arousal states. In vitro [35S]GTPγS autoradiography was performed using coronal brain stem sections from six rats. Hcrt-I (200 nM) significantly increased [35S]GTPγS binding over basal levels in locus coeruleus (24{\%}), dorsal raphe nucleus (12{\%}), pontine reticular nucleus, oral part (28{\%}), and pontine reticular nucleus, caudal part (40{\%}). This is the first study to quantify and localize hcrt-I-induced G protein activation in specific brain stem nuclei. The finding that hcrt-I stimulated [35S]GTPγS binding suggests that some hcrt receptors in pontine brain stem couple to inhibitory G proteins.",
author = "Rene Bernard and Ralph Lydic and Helen Baghdoyan",
year = "2002",
month = "3",
day = "25",
doi = "10.1097/00001756-200203250-00017",
language = "English (US)",
volume = "13",
pages = "447--450",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Hypocretin-I activates G proteins in arousal-related brainstem nuclei of rat

AU - Bernard, Rene

AU - Lydic, Ralph

AU - Baghdoyan, Helen

PY - 2002/3/25

Y1 - 2002/3/25

N2 - The hypocretin (hcrt) ligand-receptor system contributes to the maintenance of wakefulness. Hypocretin receptors are coupled to guanine nucleotide binding (G) proteins and the present study tested the hypothesis that hcrt-I would activate G proteins in rat brainstem nuclei known to regulate arousal states. In vitro [35S]GTPγS autoradiography was performed using coronal brain stem sections from six rats. Hcrt-I (200 nM) significantly increased [35S]GTPγS binding over basal levels in locus coeruleus (24%), dorsal raphe nucleus (12%), pontine reticular nucleus, oral part (28%), and pontine reticular nucleus, caudal part (40%). This is the first study to quantify and localize hcrt-I-induced G protein activation in specific brain stem nuclei. The finding that hcrt-I stimulated [35S]GTPγS binding suggests that some hcrt receptors in pontine brain stem couple to inhibitory G proteins.

AB - The hypocretin (hcrt) ligand-receptor system contributes to the maintenance of wakefulness. Hypocretin receptors are coupled to guanine nucleotide binding (G) proteins and the present study tested the hypothesis that hcrt-I would activate G proteins in rat brainstem nuclei known to regulate arousal states. In vitro [35S]GTPγS autoradiography was performed using coronal brain stem sections from six rats. Hcrt-I (200 nM) significantly increased [35S]GTPγS binding over basal levels in locus coeruleus (24%), dorsal raphe nucleus (12%), pontine reticular nucleus, oral part (28%), and pontine reticular nucleus, caudal part (40%). This is the first study to quantify and localize hcrt-I-induced G protein activation in specific brain stem nuclei. The finding that hcrt-I stimulated [35S]GTPγS binding suggests that some hcrt receptors in pontine brain stem couple to inhibitory G proteins.

UR - http://www.scopus.com/inward/record.url?scp=0037170539&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037170539&partnerID=8YFLogxK

U2 - 10.1097/00001756-200203250-00017

DO - 10.1097/00001756-200203250-00017

M3 - Article

C2 - 11930158

AN - SCOPUS:0037170539

VL - 13

SP - 447

EP - 450

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 4

ER -