Hypothermia attenuates β1 integrin expression on extravasated neutrophils in an animal model of meningitis

Mark Rowin, V. Xue, J. Irazuzta

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Brain injury in meningitis occurs in part as a consequence of leukocyte migration and activation. Leukocyte integrins are pivotal in the inflammatory response by mediating adhesion to vascular endothelium and extracellular matrix proteins. We have demonstrated that moderate hypothermia early in the course of meningitis decreases leukocyte sequestration within the brain parenchyma. This study examines whether hypothermia alters neutrophil integrin expression in a rabbit model of bacterial meningitis. Prior to the induction of meningitis, peripheral blood samples were obtained and the neutrophils isolated. Sixteen hours after inducing group B streptococcal meningitis, animals were treated with antibiotics, IV fluids, and mechanically ventilated. Animals were randomized to hypothermia (32-33°C) or normothermia conditions. After 10 hours of hypothermia or normothermia, neutrophils were isolated from the blood and cerebral spinal fluid (CSF), stained for β1 and β2 integrins, and analyzed using flow cytometry. Cerebral spinal fluid neutrophil β1 integrin expression was significantly decreased in hypothermic animals. Beta-1 integrins can assume a higher affinity or "activated" state following inflammatory stimulation. Expression of "activated" β1 integrins was also significantly decreased in hypothermic animals. Beta2 CSF neutrophil integrin expression was decreased in hypothermic animals, but failed to reach significance. These data suggest hypothermia may attenuate extravasated leukocyte expression of both total and "activated" β1 integrins.

Original languageEnglish (US)
Pages (from-to)137-144
Number of pages8
JournalInflammation
Volume25
Issue number3
DOIs
StatePublished - Jun 11 2001

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Hypothermia
Meningitis
Integrins
Neutrophils
Animal Models
Leukocytes
Integrin beta Chains
Bacterial Meningitides
Extracellular Matrix Proteins
Vascular Endothelium
Brain Injuries
Flow Cytometry
Anti-Bacterial Agents
Rabbits
Brain

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Hypothermia attenuates β1 integrin expression on extravasated neutrophils in an animal model of meningitis. / Rowin, Mark; Xue, V.; Irazuzta, J.

In: Inflammation, Vol. 25, No. 3, 11.06.2001, p. 137-144.

Research output: Contribution to journalArticle

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