Identification and characterization of a functional human Ig VλVI germline gene

Ch'ang Lan-Yang, Yen Chin-Ping, Linda Besl, Maria Schell, Alan Solomon

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have isolated from a human genomic library a potentially functional and distinctive germline gene, designated IGLV6S1, that encodes for light chains of the VλVI subgroup. An identical germline gene was cloned from fibroblasts obtained from a patient with light-chain-associated amyloidosis (AL amyloidosis) whose serum and urine contained, respectively, a monoclonal IgGλvi protein and a λvi Bence Jones protein. Isolation and characterization of cDNA cloned from the patient's bone marrow-derived monoclonal plasma cells revealed that the nucleotide and predicted protein sequences of the rearranged gene were ∼95% and ∼90% homologous to those of the germline gene, respectively. The finding that the transcriptional start site for λvi RNA synthesis was located upstream of the putative TATA-box promoter, rather than downstream as found for the VλII subgroup, implies that a different transcriptional machinery controls the expression of the human VλVI-gene family.

Original languageEnglish (US)
Pages (from-to)531-536
Number of pages6
JournalMolecular Immunology
Volume31
Issue number7
DOIs
StatePublished - Jan 1 1994

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Genes
Bence Jones Protein
Light
TATA Box
Genomic Library
Amyloidosis
Plasma Cells
Proteins
Nucleotides
Complementary DNA
Fibroblasts
Bone Marrow
Urine
RNA
Serum

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Identification and characterization of a functional human Ig VλVI germline gene. / Lan-Yang, Ch'ang; Chin-Ping, Yen; Besl, Linda; Schell, Maria; Solomon, Alan.

In: Molecular Immunology, Vol. 31, No. 7, 01.01.1994, p. 531-536.

Research output: Contribution to journalArticle

Lan-Yang, Ch'ang ; Chin-Ping, Yen ; Besl, Linda ; Schell, Maria ; Solomon, Alan. / Identification and characterization of a functional human Ig VλVI germline gene. In: Molecular Immunology. 1994 ; Vol. 31, No. 7. pp. 531-536.
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