Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain

Marta C. Nunes, Neeta Roy, H. Michael Keyoung, Robert R. Goodman, Guy McKhann, Li Jiang, Jian Kang, Maiken Nedergaard, Steven A. Goldman

Research output: Contribution to journalArticle

563 Citations (Scopus)

Abstract

The subcortical white matter of the adult human brain harbors a pool of glial progenitor cells. These cells can be isolated by fluorescence-activated cell sorting (FACS) after either transfection with green fluorescent protein (GFP) under the control of the CNP2 promoter, or A2B5-targeted immunotagging. Although these cells give rise largely to oligodendrocytes, in low-density culture we observed that some also generated neurons. We thus asked whether these nominally glial progenitors might include multipotential progenitor cells capable of neurogenesis. We found that adult human white-matter progenitor cells (WMPCs) could be passaged as neurospheres in vitro and that these cells generated functionally competent neurons and glia both in vitro and after xenograft to the fetal rat brain. WMPCs were able to produce neurons after their initial isolation and did not require in vitro expansion or reprogramming to do so. These experiments indicate that an abundant pool of mitotically competent neurogenic progenitor cells resides in the adult human white matter.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalNature Medicine
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2003
Externally publishedYes

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Neurons
Brain
Stem Cells
Neuroglia
Ports and harbors
Green Fluorescent Proteins
Sorting
Heterografts
Rats
Fluorescence
Cells
Neurogenesis
Oligodendroglia
Transfection
Flow Cytometry
White Matter
Experiments
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. / Nunes, Marta C.; Roy, Neeta; Keyoung, H. Michael; Goodman, Robert R.; McKhann, Guy; Jiang, Li; Kang, Jian; Nedergaard, Maiken; Goldman, Steven A.

In: Nature Medicine, Vol. 9, No. 4, 01.04.2003, p. 439-447.

Research output: Contribution to journalArticle

Nunes, MC, Roy, N, Keyoung, HM, Goodman, RR, McKhann, G, Jiang, L, Kang, J, Nedergaard, M & Goldman, SA 2003, 'Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain', Nature Medicine, vol. 9, no. 4, pp. 439-447. https://doi.org/10.1038/nm837
Nunes, Marta C. ; Roy, Neeta ; Keyoung, H. Michael ; Goodman, Robert R. ; McKhann, Guy ; Jiang, Li ; Kang, Jian ; Nedergaard, Maiken ; Goldman, Steven A. / Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain. In: Nature Medicine. 2003 ; Vol. 9, No. 4. pp. 439-447.
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