Identification and quantification of hemoglobins A2 and Barts with an enzyme-labeled immunosorbent assay

F. A. Garver, H. Singh, H. Moscoso, Daniel Kestler, B. S. McGuire

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Abstract

The presence and quantity of hemoglobins Barts and A2 in hemolysates from normal donors and individuals with α- and β-thalassemia trait, respectively, were determined with an enzyme-labeled immunosorbent assay (ELISA). This technique requires the incorporation of monospecific antisera capable of specifically reacting only with these hemoglobins, e.g., with the δ chain of Hb A2 and γ4 chains of Hb Barts. By the ELISA, the mean percentage of Hb Barts in hemolysates from normal persons and persons with α-thalassemia was 0.25 (SD 0.07) and 6.1 (SD 0.40), respectively. Corresponding values for Hb A2 in hemolysates from normals and persons with β-thalassemia were 3.1 (SD 0.22) and 5.9 (SD 0.21), respectively. The results obtained by the ELISA procedure were in good agreement with those determined by radioimmunoassay or microcolumn chromatography. The ELISA technique is more sensitive and specific than biochemical assays currently used to measure these hemoglobins and can detect 250 ng of Hb in Barts in 100 μg of hemoglobin or 50 ng of Hb A2 in 5 μg of hemoglobin.

Original languageEnglish (US)
Pages (from-to)1205-1208
Number of pages4
JournalClinical Chemistry
Volume30
Issue number7
StatePublished - Dec 1 1984

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Hemoglobin A2
Immunosorbents
Thalassemia
Assays
Hemoglobins
Enzymes
Immunosorbent Techniques
Radioimmunoassay
Chromatography
Immune Sera
hemoglobin Bart's
hemoglobin A2'

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Identification and quantification of hemoglobins A2 and Barts with an enzyme-labeled immunosorbent assay. / Garver, F. A.; Singh, H.; Moscoso, H.; Kestler, Daniel; McGuire, B. S.

In: Clinical Chemistry, Vol. 30, No. 7, 01.12.1984, p. 1205-1208.

Research output: Contribution to journalArticle

Garver, F. A. ; Singh, H. ; Moscoso, H. ; Kestler, Daniel ; McGuire, B. S. / Identification and quantification of hemoglobins A2 and Barts with an enzyme-labeled immunosorbent assay. In: Clinical Chemistry. 1984 ; Vol. 30, No. 7. pp. 1205-1208.
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