Identification of a novel Mr = 76-kDa form of beta-adrenergic receptors

Suleiman Bahouth, Lucky K. Kelley, Carl H. Smith, Marty A. Arbabian, Arnold E. Ruoho, Craig C. Malbon

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The structure of the human β-adrenergic receptor in purified basal membranes of human placental syncytiotrophoblast was probed using photoaffinity labeling. Basal membranes display a high specific activity of receptors (4-5 pmol/mg protein) and possess both β1- and β2-adrenergic receptors subtypes. Autoradioraphy of membranes that were incubated with the β-adrenergic antagonist [125I]iodoazidobenzylpindolol, photolyzed and then subjected to sodium dodecylsulfate-polyacrylamide gel electrophoresis, identified four radiolabeled peptides. Mr = 65-kDa, 54-kDa, 43-kDa and a novel higher molecular weight 76-kDa form of the receptor. Photoaffinity labeling of each of these four peptides displayed the pharmacological properties expected for true β-adrenergic receptors. The 76-kDa photoaffinity labeled receptor peptide observed in human placenta basal membranes has not been reported elsewhere. Competition studies with the β1-selective ligand CGP-20712A demonstrate that the photoaffinity labeled receptor peptides are composed of both β1- and β2-adrenergic receptor subtypes.

Original languageEnglish (US)
Pages (from-to)411-417
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume141
Issue number2
DOIs
StatePublished - Dec 15 1986
Externally publishedYes

Fingerprint

Receptors, Adrenergic, beta
Adrenergic Receptors
Membranes
Peptide Receptors
Labeling
Peptides
Adrenergic Antagonists
Trophoblasts
Electrophoresis
Placenta
Polyacrylamide Gel Electrophoresis
Molecular Weight
Sodium
Molecular weight
Pharmacology
Ligands
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of a novel Mr = 76-kDa form of beta-adrenergic receptors. / Bahouth, Suleiman; Kelley, Lucky K.; Smith, Carl H.; Arbabian, Marty A.; Ruoho, Arnold E.; Malbon, Craig C.

In: Biochemical and Biophysical Research Communications, Vol. 141, No. 2, 15.12.1986, p. 411-417.

Research output: Contribution to journalArticle

Bahouth, Suleiman ; Kelley, Lucky K. ; Smith, Carl H. ; Arbabian, Marty A. ; Ruoho, Arnold E. ; Malbon, Craig C. / Identification of a novel Mr = 76-kDa form of beta-adrenergic receptors. In: Biochemical and Biophysical Research Communications. 1986 ; Vol. 141, No. 2. pp. 411-417.
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