Identification of a RAPD marker linked to progressive rod-cone degeneration in dogs

Weikuan Gu, Gregory M. Acland, Amelia A. Langston, Elaine A. Ostrander, Gustavo D. Aguirre, Kunal Ray

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Random amplified polymorphic DNA (RAPD) analysis has been used widely in plant and fungi for identification of markers linked to genetic traits and mapping, but its use is limited to identification of intra- and inter- species difference in domestic mammals. We report here identification of a RAPD-derived marker linked to progressive rod-cone degeneration (prcd), an inherited autosomal recessive retinal disease of dogs. A total of 400 standard 10-mer primers were used for amplification by use of DNA samples from normal (+/+) and affected (prcd/prcd) dogs. A single primer was identified which amplified a 1.5-kb DNA fragment only from normal dogs. PCR with longer primers designed from the sequence-characterized amplified region of the 1.5-kb DNA fragment identified a co-dominant multi-allelic polymorphism in the prcd-informative pedigree. Three recombinants were identified among 34 informative offsprings, yielding a LOD score of 5.568 at θ = 0.091. This marker was mapped to two canine-rodent hybrid cell lines in which two genes (canine homologues of human breast cancer 1 susceptibility gene, and cGMP phosphodiesterase γ-subunit gene), and three anonymous microsatellites have been identified. This is the first reported identification of a RAPD-derived marker with multiple alleles linked to a mammalian disease locus.

Original languageEnglish (US)
Pages (from-to)740-744
Number of pages5
JournalMammalian Genome
Volume9
Issue number9
DOIs
StatePublished - Sep 15 1998
Externally publishedYes

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Genetic Markers
Dogs
DNA
Canidae
Genes
Retinal Diseases
Hybrid Cells
Phosphoric Diester Hydrolases
Pedigree
Microsatellite Repeats
Mammals
Rodentia
Fungi
Alleles
Breast Neoplasms
Cell Line
Polymerase Chain Reaction
Cone-Rod Dystrophies

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Gu, W., Acland, G. M., Langston, A. A., Ostrander, E. A., Aguirre, G. D., & Ray, K. (1998). Identification of a RAPD marker linked to progressive rod-cone degeneration in dogs. Mammalian Genome, 9(9), 740-744. https://doi.org/10.1007/s003359900855

Identification of a RAPD marker linked to progressive rod-cone degeneration in dogs. / Gu, Weikuan; Acland, Gregory M.; Langston, Amelia A.; Ostrander, Elaine A.; Aguirre, Gustavo D.; Ray, Kunal.

In: Mammalian Genome, Vol. 9, No. 9, 15.09.1998, p. 740-744.

Research output: Contribution to journalArticle

Gu, W, Acland, GM, Langston, AA, Ostrander, EA, Aguirre, GD & Ray, K 1998, 'Identification of a RAPD marker linked to progressive rod-cone degeneration in dogs', Mammalian Genome, vol. 9, no. 9, pp. 740-744. https://doi.org/10.1007/s003359900855
Gu, Weikuan ; Acland, Gregory M. ; Langston, Amelia A. ; Ostrander, Elaine A. ; Aguirre, Gustavo D. ; Ray, Kunal. / Identification of a RAPD marker linked to progressive rod-cone degeneration in dogs. In: Mammalian Genome. 1998 ; Vol. 9, No. 9. pp. 740-744.
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