Identification of an amelin isoform located in axons

Warren E. Zimmer, Ian S. Zagon, Linda A. Casoria, Steven Goodman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A new axonal isoform of amelin, an analogue of the erythrocyte spectrin binding protein termed protein 4.1 has been identified in mouse brain. This new isoform has a molecular weight of 93 kDa, and migrates to a more acidic pH (pH 7.5-8.0) than the previously described amelin E (pH 8.5) on two dimensional NEPHGE-SDS PAGE. The 93 kDa protein looks nearly identical to amelin E on two dimensional chymotryptic iodopeptide mapping, and both share partial homology with rbc protein 4.1. The new isoform is located in axons, and the soma of neurons in mouse cerebellum, while amelin E is located in neuronal soma and dendrites. The axonal amelin antibody detects a 97 kDa protein in embryonic tissue which diminishes during development; and a 93 kDa protein which is first seen at postnatal day 1 of mouse brain ontogeny, increasing constantly to its adult concentrations. This time course of expression is quite different than amelin E, which is present at embryonic day 15 and diminishes constantly reaching its lowest concentration in the adult brain. We hypothesize that axonal amelin and amelin E may play important roles in the interaction of brain spectrin(240/235) and brain spectrin (240/235E) with f-actin and neuronal membranes.

Original languageEnglish (US)
Pages (from-to)94-100
Number of pages7
JournalBrain Research
Volume582
Issue number1
DOIs
StatePublished - Jun 5 1992
Externally publishedYes

Fingerprint

Axons
Protein Isoforms
Brain
Spectrin
Carisoprodol
Proteins
Iodoproteins
Dendrites
Cerebellum
Actins
Polyacrylamide Gel Electrophoresis
Erythrocytes
Molecular Weight
Neurons
Membranes
Antibodies

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Identification of an amelin isoform located in axons. / Zimmer, Warren E.; Zagon, Ian S.; Casoria, Linda A.; Goodman, Steven.

In: Brain Research, Vol. 582, No. 1, 05.06.1992, p. 94-100.

Research output: Contribution to journalArticle

Zimmer, Warren E. ; Zagon, Ian S. ; Casoria, Linda A. ; Goodman, Steven. / Identification of an amelin isoform located in axons. In: Brain Research. 1992 ; Vol. 582, No. 1. pp. 94-100.
@article{fa22dc4c70b848d894b2fdbdcda985c2,
title = "Identification of an amelin isoform located in axons",
abstract = "A new axonal isoform of amelin, an analogue of the erythrocyte spectrin binding protein termed protein 4.1 has been identified in mouse brain. This new isoform has a molecular weight of 93 kDa, and migrates to a more acidic pH (pH 7.5-8.0) than the previously described amelin E (pH 8.5) on two dimensional NEPHGE-SDS PAGE. The 93 kDa protein looks nearly identical to amelin E on two dimensional chymotryptic iodopeptide mapping, and both share partial homology with rbc protein 4.1. The new isoform is located in axons, and the soma of neurons in mouse cerebellum, while amelin E is located in neuronal soma and dendrites. The axonal amelin antibody detects a 97 kDa protein in embryonic tissue which diminishes during development; and a 93 kDa protein which is first seen at postnatal day 1 of mouse brain ontogeny, increasing constantly to its adult concentrations. This time course of expression is quite different than amelin E, which is present at embryonic day 15 and diminishes constantly reaching its lowest concentration in the adult brain. We hypothesize that axonal amelin and amelin E may play important roles in the interaction of brain spectrin(240/235) and brain spectrin (240/235E) with f-actin and neuronal membranes.",
author = "Zimmer, {Warren E.} and Zagon, {Ian S.} and Casoria, {Linda A.} and Steven Goodman",
year = "1992",
month = "6",
day = "5",
doi = "10.1016/0006-8993(92)90321-Y",
language = "English (US)",
volume = "582",
pages = "94--100",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Identification of an amelin isoform located in axons

AU - Zimmer, Warren E.

AU - Zagon, Ian S.

AU - Casoria, Linda A.

AU - Goodman, Steven

PY - 1992/6/5

Y1 - 1992/6/5

N2 - A new axonal isoform of amelin, an analogue of the erythrocyte spectrin binding protein termed protein 4.1 has been identified in mouse brain. This new isoform has a molecular weight of 93 kDa, and migrates to a more acidic pH (pH 7.5-8.0) than the previously described amelin E (pH 8.5) on two dimensional NEPHGE-SDS PAGE. The 93 kDa protein looks nearly identical to amelin E on two dimensional chymotryptic iodopeptide mapping, and both share partial homology with rbc protein 4.1. The new isoform is located in axons, and the soma of neurons in mouse cerebellum, while amelin E is located in neuronal soma and dendrites. The axonal amelin antibody detects a 97 kDa protein in embryonic tissue which diminishes during development; and a 93 kDa protein which is first seen at postnatal day 1 of mouse brain ontogeny, increasing constantly to its adult concentrations. This time course of expression is quite different than amelin E, which is present at embryonic day 15 and diminishes constantly reaching its lowest concentration in the adult brain. We hypothesize that axonal amelin and amelin E may play important roles in the interaction of brain spectrin(240/235) and brain spectrin (240/235E) with f-actin and neuronal membranes.

AB - A new axonal isoform of amelin, an analogue of the erythrocyte spectrin binding protein termed protein 4.1 has been identified in mouse brain. This new isoform has a molecular weight of 93 kDa, and migrates to a more acidic pH (pH 7.5-8.0) than the previously described amelin E (pH 8.5) on two dimensional NEPHGE-SDS PAGE. The 93 kDa protein looks nearly identical to amelin E on two dimensional chymotryptic iodopeptide mapping, and both share partial homology with rbc protein 4.1. The new isoform is located in axons, and the soma of neurons in mouse cerebellum, while amelin E is located in neuronal soma and dendrites. The axonal amelin antibody detects a 97 kDa protein in embryonic tissue which diminishes during development; and a 93 kDa protein which is first seen at postnatal day 1 of mouse brain ontogeny, increasing constantly to its adult concentrations. This time course of expression is quite different than amelin E, which is present at embryonic day 15 and diminishes constantly reaching its lowest concentration in the adult brain. We hypothesize that axonal amelin and amelin E may play important roles in the interaction of brain spectrin(240/235) and brain spectrin (240/235E) with f-actin and neuronal membranes.

UR - http://www.scopus.com/inward/record.url?scp=0026724839&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026724839&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(92)90321-Y

DO - 10.1016/0006-8993(92)90321-Y

M3 - Article

VL - 582

SP - 94

EP - 100

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -