Identification of Broadly Reactive Continuous Antigenic Determinants of Simian Immunodeficiency Virus Glycoproteins

Mark Miller, Michael Murphey-Corb, Ronald C. Montelaro

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The env polyprotein sequences of several simian immunodeficiency virus (SIV) isolates were analyzed using computer algorithms designed to predict immunologically reactive protein segments. Peptides corresponding to predicted epitopes were synthesized and employed in peptide enzyme-linked immunosorbant assay (ELISA) screening of serum panels from experimentally infected macaques, as well as naturally infected, asymptomatic mangabeys and African green monkeys. Several of the peptides are recognized by a high percentage of antisera from each panel of monkeys indicating that they represent group-specific antigenic determinants of SIV. Several type-specific determinants also were identified. These peptides may be a useful tool for studying the kinetics of SIV glycoprotein-specific immune responses produced by infected and vaccine-protected monkeys at the epitope level.

Original languageEnglish (US)
Pages (from-to)1153-1164
Number of pages12
JournalAIDS Research and Human Retroviruses
Volume8
Issue number6
DOIs
StatePublished - Jan 1 1992

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Simian Immunodeficiency Virus
Epitopes
Glycoproteins
Peptides
Haplorhini
Cercocebus
env Gene Products
Cercopithecus aethiops
Macaca
Immune Sera
Vaccines
Enzymes
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Infectious Diseases
  • Virology

Cite this

Identification of Broadly Reactive Continuous Antigenic Determinants of Simian Immunodeficiency Virus Glycoproteins. / Miller, Mark; Murphey-Corb, Michael; Montelaro, Ronald C.

In: AIDS Research and Human Retroviruses, Vol. 8, No. 6, 01.01.1992, p. 1153-1164.

Research output: Contribution to journalArticle

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