Identification of p53 Mutations in Endometrial Adenocarcinoma by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism

Stephen S. Wachtel, Gwendolyn Wachtel, Lee P. Shulman, Owen Phillips, Brigitte Miller, Guy Photopulos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

OBJECTIVE: We determined whether mutations in p53 exons 5-6-7-8, as detected in the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) test, might be correlated with stage or grade in endometrial adenocarcinoma. METHODS: We amplified sequences containing exons 5, 6, 7, or 8 in DNA from tumors and controls. Mutation within the amplified sequences was indicated by changes in electrophoretic mobility (band shifts) in the SSCP test. The results were analyzed statistically and compared with the results of other, similar studies. RESULTS: We identified 15 band shifts among 47 endometrial tumors studied (band shifts in two different exons in two cases) and none among 42 controls. Band shifts in exons 5 and 8 were associated uniformly with grade 2 or grade 3 histology. In other studies p53 mutations were correlated with advanced-stage malignancy. CONCLUSION: Further evaluation of particular p53 mutations and their relation to disease course in endometrial adenocarcinoma seems warranted. The PCR-SSCP test seems well-suited to this purpose. (J Soc Gynecol Invest 1994 ;1 :234-7).

Original languageEnglish (US)
Pages (from-to)234-237
Number of pages4
JournalJournal of the Society for Gynecologic Investigation
Volume1
Issue number3
DOIs
StatePublished - Jan 1 1994

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Exons
Adenocarcinoma
Polymerase Chain Reaction
Mutation
Single-Stranded Conformational Polymorphism
Neoplasms
Histology
DNA

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynecology

Cite this

Identification of p53 Mutations in Endometrial Adenocarcinoma by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism. / Wachtel, Stephen S.; Wachtel, Gwendolyn; Shulman, Lee P.; Phillips, Owen; Miller, Brigitte; Photopulos, Guy.

In: Journal of the Society for Gynecologic Investigation, Vol. 1, No. 3, 01.01.1994, p. 234-237.

Research output: Contribution to journalArticle

Wachtel, Stephen S. ; Wachtel, Gwendolyn ; Shulman, Lee P. ; Phillips, Owen ; Miller, Brigitte ; Photopulos, Guy. / Identification of p53 Mutations in Endometrial Adenocarcinoma by Polymerase Chain Reaction-Single-Strand Conformation Polymorphism. In: Journal of the Society for Gynecologic Investigation. 1994 ; Vol. 1, No. 3. pp. 234-237.
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AU - Phillips, Owen

AU - Miller, Brigitte

AU - Photopulos, Guy

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N2 - OBJECTIVE: We determined whether mutations in p53 exons 5-6-7-8, as detected in the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) test, might be correlated with stage or grade in endometrial adenocarcinoma. METHODS: We amplified sequences containing exons 5, 6, 7, or 8 in DNA from tumors and controls. Mutation within the amplified sequences was indicated by changes in electrophoretic mobility (band shifts) in the SSCP test. The results were analyzed statistically and compared with the results of other, similar studies. RESULTS: We identified 15 band shifts among 47 endometrial tumors studied (band shifts in two different exons in two cases) and none among 42 controls. Band shifts in exons 5 and 8 were associated uniformly with grade 2 or grade 3 histology. In other studies p53 mutations were correlated with advanced-stage malignancy. CONCLUSION: Further evaluation of particular p53 mutations and their relation to disease course in endometrial adenocarcinoma seems warranted. The PCR-SSCP test seems well-suited to this purpose. (J Soc Gynecol Invest 1994 ;1 :234-7).

AB - OBJECTIVE: We determined whether mutations in p53 exons 5-6-7-8, as detected in the polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) test, might be correlated with stage or grade in endometrial adenocarcinoma. METHODS: We amplified sequences containing exons 5, 6, 7, or 8 in DNA from tumors and controls. Mutation within the amplified sequences was indicated by changes in electrophoretic mobility (band shifts) in the SSCP test. The results were analyzed statistically and compared with the results of other, similar studies. RESULTS: We identified 15 band shifts among 47 endometrial tumors studied (band shifts in two different exons in two cases) and none among 42 controls. Band shifts in exons 5 and 8 were associated uniformly with grade 2 or grade 3 histology. In other studies p53 mutations were correlated with advanced-stage malignancy. CONCLUSION: Further evaluation of particular p53 mutations and their relation to disease course in endometrial adenocarcinoma seems warranted. The PCR-SSCP test seems well-suited to this purpose. (J Soc Gynecol Invest 1994 ;1 :234-7).

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