Identification of protein kinase C-isoforms involved in κ-opioid- and α-adrenergic receptor dependent cardioprotection

W. G. Pyle, Polly Hofmann

Research output: Contribution to journalArticle

Abstract

We have previously shown that activation of the κ-opioid- and α-adrenergic receptors improves postischemic contractile function in the rat heart, concomitant with a reduction in both Ca2+-dependent actomyosin MgATPase activity and maximum velocity of unloaded shortening in ventricular myocytes (Biophys J. 72: 1997). We have recently reported that the protein kinase C (PKC) inhibitor chelerythrine chloride and the type I and IIa phosphatase inhibitor cantharidin block κ-opioid receptor-dependent cardioprotection (Biophys J. 74: 1998). These findings are consistent with the hypothesis that κ-opioid- and α-adrenergic receptor-PKC mediated modification of myofibrillar proteins reduce actin-myosin ATP consumption and this conservation of ATP contributes to cardioprotection during ischemia. To determine the PKC-isoforms responsible for κ-opioid- and α-adrenergic receptor-dependent cardioprotection in the rat we analyzed cytosolic, membrane, and myofibrillar extracts by Western blotting following agonist stimulation of ventricular myocytes. Confocal microscopy was used to confirm PKC translocation to myofibrils in isolated ventricular myocytes. Preliminary results show that treatment of ventricular myocytes with the PKC-activator phorbol 12-myristate acetate or the α-adrenergic agonist phenylephrine causes an increase in PKC-ε associated with the myofibrils. Further studies will be conducted to investigate the effects of κ-opioid receptor stimulation and PKC activation.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

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narcotics
adrenergic receptors
protein kinase C
Opioid Receptors
Adrenergic Receptors
Protein Kinase C
Opioid Analgesics
Protein Isoforms
myocytes
Muscle Cells
Myofibrils
myofibrils
Rats
Adenosine Triphosphate
Cantharidin
Chemical activation
cantharidin
adrenergic agonists
Actomyosin
Adrenergic Agonists

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Identification of protein kinase C-isoforms involved in κ-opioid- and α-adrenergic receptor dependent cardioprotection. / Pyle, W. G.; Hofmann, Polly.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

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