Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach

Kaushik Kumar Dey, Ipsita Pal, Rashmi Bharti, Goutam Dey, Prashanth Kumar Bhusetty Nagesh, Shashi Rajput, Aditya Parekh, Sheetal Parida, Priyanka Halder, Indranil Kulavi, Mahitosh Mandal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Despite the recent advances in diagnostic and therapeutic strategies, oral squamous cell carcinoma (OSCC) remains a major health burden. Protein biomarker discovery for early detection will help to improve patient survival rate in OSCC. Mass spectrometry-based proteomics has emerged as an excellent approach for detection of protein biomarkers in various types of cancers. In the current study, we have used 4-Plex isobaric tags for relative and absolute quantitation (iTRAQ)-based shotgun quantitative proteomic approach to identify proteins that are differentially expressed in cancerous tissues compared to normal tissues. The high-resolution mass spectrometric analysis resulted in identifying 2,074 proteins, among which 288 proteins were differentially expressed. Further, it was noticed that 162 proteins were upregulated, while 125 proteins were downregulated in OSCC-derived cancer tissue samples as compared to the adjacent normal tissues. We identified some of the known molecules which were reported earlier in OSCC such as MMP-9 (8.4-fold), ZNF142 (5.6-fold), and S100A7 (3.5-fold). Apart from this, we have also identified some novel signature proteins which have not been reported earlier in OSCC including ras-related protein Rab-2A isoform, RAB2A (4.6-fold), and peroxiredoxin-1, PRDX1 (2.2-fold). The immunohistochemistry-based validation using tissue microarray slides in OSCC revealed overexpression of the RAB2A and PRDX1 gene in 80 and 68 % of the tested clinical cases, respectively. This study will not only serve as a resource of candidate biomarkers but will contribute towards the existing knowledge on the role of the candidate molecules towards disease progression and therapeutic potential.

Original languageEnglish (US)
Pages (from-to)9829-9837
Number of pages9
JournalTumor Biology
Volume36
Issue number12
DOIs
StatePublished - Dec 1 2015

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Proteomics
Squamous Cell Carcinoma
Mass Spectrometry
Biomarkers
Proteins
Peroxiredoxins
ras Proteins
Firearms
Matrix Metalloproteinases
Disease Progression
Neoplasms
Protein Isoforms
Down-Regulation
Survival Rate
Immunohistochemistry
Health
Therapeutics
Genes

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach. / Dey, Kaushik Kumar; Pal, Ipsita; Bharti, Rashmi; Dey, Goutam; Bhusetty Nagesh, Prashanth Kumar; Rajput, Shashi; Parekh, Aditya; Parida, Sheetal; Halder, Priyanka; Kulavi, Indranil; Mandal, Mahitosh.

In: Tumor Biology, Vol. 36, No. 12, 01.12.2015, p. 9829-9837.

Research output: Contribution to journalArticle

Dey, Kaushik Kumar ; Pal, Ipsita ; Bharti, Rashmi ; Dey, Goutam ; Bhusetty Nagesh, Prashanth Kumar ; Rajput, Shashi ; Parekh, Aditya ; Parida, Sheetal ; Halder, Priyanka ; Kulavi, Indranil ; Mandal, Mahitosh. / Identification of RAB2A and PRDX1 as the potential biomarkers for oral squamous cell carcinoma using mass spectrometry-based comparative proteomic approach. In: Tumor Biology. 2015 ; Vol. 36, No. 12. pp. 9829-9837.
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