Identification of secretion determinants of the Bordotella pertussis BrkA autotransporter

David C. Oliver, George Huang, Rachel C. Fernandez

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The autotransporters comprise a functionally diverse family of gram-negative proteins that mediate their own export across the bacterial outer membrane. They consist of an amino-terminal passenger region called the "α-domain" and the structural hallmark of the autotransporter family, a carboxy-terminal transporter region usually referred to as the "β-domain." The passenger region can be quite diverse and constitutes the effector functions of these proteins, whereas the C-terminal region is conserved and is responsible for translocating the passenger moiety across the outer membrane. BrkA is the 103-kDa autotransporter protein in Bordetella pertussis that is cleaved to yield a 73-kDa N-terminal α-domain and a 30-kDa C-terminal β-domain. We have previously shown that a recombinant form of the β-domain of BrkA is capable of forming channels in artificial membranes. Here, we define two additional secretion determinants of BrkA. N-terminal sequencing of the 73-kDa BrkA passenger from B. pertussis and Escherichia coli revealed that BrkA has a 42-amino-acid signal peptide. In addition, deletion analysis of BrkA identified a 31- to 39-amino-acid region found immediately upstream of the β-domain that was essential for surface expression. This 31- to 39-amino-acid linker region, together with the β-domain, defines the minimal BrkA translocation unit. The linker region may also serve to anchor the BrkA passenger to the bacterial surface.

Original languageEnglish (US)
Pages (from-to)489-495
Number of pages7
JournalJournal of bacteriology
Volume185
Issue number2
DOIs
StatePublished - Jan 1 2003

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Whooping Cough
Bordetella pertussis
Amino Acids
Artificial Membranes
Membranes
Protein Sorting Signals
Protein C
Proteins
Escherichia coli
Type V Secretion Systems

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Identification of secretion determinants of the Bordotella pertussis BrkA autotransporter. / Oliver, David C.; Huang, George; Fernandez, Rachel C.

In: Journal of bacteriology, Vol. 185, No. 2, 01.01.2003, p. 489-495.

Research output: Contribution to journalArticle

Oliver, David C. ; Huang, George ; Fernandez, Rachel C. / Identification of secretion determinants of the Bordotella pertussis BrkA autotransporter. In: Journal of bacteriology. 2003 ; Vol. 185, No. 2. pp. 489-495.
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