Identification of the Candida albicans Cap1p regulon

Sadri Znaidi, Katherine S. Barker, Sandra Weber, Anne Marie Alarco, Teresa T. Liu, Geneviève Boucher, Phillip Rogers, Martine Raymond

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Cap1p, a transcription factor of the basic region leucine zipper family, regulates the oxidative stress response (OSR) in Candida albicans. Alteration of its C-terminal cysteine-rich domain (CRD) results in Cap1p nuclear retention and transcriptional activation. To better understand the function of Cap1p in C. albicans, we used genome-wide location profiling (chromatin immunoprecipitation-on-chip) to identify its transcriptional targets in vivo. A triple-hemagglutinin (HA 3) epitope was introduced at the C terminus of wild-type Cap1p (Cap1p-HA 3) or hyperactive Cap1p with an altered CRD (Cap1p-CSE-HA 3). Location profiling using wholegenome oligonucleotide tiling microarrays identified 89 targets bound by Cap1p-HA 3 or Caplp-CSE-HA 3 (the binding ratio was at least twofold; P ≤0.01). Strikingly, Cap1p binding was detected not only at the promoter region of its target genes but also at their 3' ends and within their open reading frames, suggesting that Cap1p may associate with the transcriptional or chromatin remodeling machinery to exert its activity. Overrepresented functional groups of the Cap1p targets (P ≤ 0.02) included 11 genes involved in the OSR (CAP1, GLR1, TRX1, SOD1, CAT1, and others), 13 genes involved in response to drugs (PDR16, MDR1, FLU1, YCF1, FCR1, and others), 4 genes involved in phospholipid transport (PDR16, GIT1, RTA2, and orfl9.932), and 3 genes involved in the regulation of nitrogen utilization (GST3, orfl9.2693, and orfl9.3121), suggesting that Cap1p has other cellular functions in addition to the OSR. Bioinformatic analyses of the bound sequences suggest that Cap1p recognizes the DNA motif 5'-MTKASTMA. Finally, transcriptome analyses showed that increased expression generally accompanies Cap1p binding at its targets, indicating that Cap1p functions as a transcriptional activator.

Original languageEnglish (US)
Pages (from-to)806-820
Number of pages15
JournalEukaryotic Cell
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2009

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Regulon
Candida albicans
Oxidative Stress
Genes
Cysteine
Basic-Leucine Zipper Transcription Factors
Nucleotide Motifs
Chromatin Assembly and Disassembly
Chromatin Immunoprecipitation
Hemagglutinins
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Computational Biology
Genetic Promoter Regions
Transcriptional Activation
Open Reading Frames
Sequence Analysis
Epitopes
Phospholipids
Nitrogen

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Znaidi, S., Barker, K. S., Weber, S., Alarco, A. M., Liu, T. T., Boucher, G., ... Raymond, M. (2009). Identification of the Candida albicans Cap1p regulon. Eukaryotic Cell, 8(6), 806-820. https://doi.org/10.1128/EC.00002-09

Identification of the Candida albicans Cap1p regulon. / Znaidi, Sadri; Barker, Katherine S.; Weber, Sandra; Alarco, Anne Marie; Liu, Teresa T.; Boucher, Geneviève; Rogers, Phillip; Raymond, Martine.

In: Eukaryotic Cell, Vol. 8, No. 6, 01.06.2009, p. 806-820.

Research output: Contribution to journalArticle

Znaidi, S, Barker, KS, Weber, S, Alarco, AM, Liu, TT, Boucher, G, Rogers, P & Raymond, M 2009, 'Identification of the Candida albicans Cap1p regulon', Eukaryotic Cell, vol. 8, no. 6, pp. 806-820. https://doi.org/10.1128/EC.00002-09
Znaidi S, Barker KS, Weber S, Alarco AM, Liu TT, Boucher G et al. Identification of the Candida albicans Cap1p regulon. Eukaryotic Cell. 2009 Jun 1;8(6):806-820. https://doi.org/10.1128/EC.00002-09
Znaidi, Sadri ; Barker, Katherine S. ; Weber, Sandra ; Alarco, Anne Marie ; Liu, Teresa T. ; Boucher, Geneviève ; Rogers, Phillip ; Raymond, Martine. / Identification of the Candida albicans Cap1p regulon. In: Eukaryotic Cell. 2009 ; Vol. 8, No. 6. pp. 806-820.
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