Identification of the segments of the mouse transferrin receptor 1 required for mouse mammary tumor virus infection

Enxiu Wang, Lorraine Albritton, Susan R. Ross

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Most enveloped viruses enter cells through binding of virion surface envelope proteins to receptors found on the plasma membrane of the cell. The beta retrovirus mouse mammary tumor virus (MMTV) uses transferrin receptor 1 (TfR1) to enter cells in a pH-dependent mechanism, probably co-trafficking with TfR1 to an acidic compartment where virus entry occurs. We have shown here that, although mouse and rat TfR1 function as entry receptors, cat, dog, hamster, or human TfR1s do not support MMTV infection. We also demonstrated that MMTV entry is independent of transferrin, iron, and the TfR1 cofactor hereditary hematochromatosis HFE protein. Using chimeric mouse/human hybrid TfR1 constructs, we determined the site of interaction with MMTV and found that it maps to two segments physically disparate from the TfR and HFE binding sites. Thus, MMTV has apparently evolved to enter cells independently of the iron status of the host.

Original languageEnglish (US)
Pages (from-to)10243-10249
Number of pages7
JournalJournal of Biological Chemistry
Volume281
Issue number15
DOIs
StatePublished - Apr 14 2006

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Tumor Virus Infections
Mouse mammary tumor virus
Viruses
Tumors
Transferrin Receptors
Virus Internalization
Iron
Retroviridae
Transferrin
Plasma Cells
Cricetinae
Virion
Membrane Proteins
Cats
Cell membranes
Binding Sites
Cell Membrane
Mouse Tfrc protein
Dogs
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Identification of the segments of the mouse transferrin receptor 1 required for mouse mammary tumor virus infection. / Wang, Enxiu; Albritton, Lorraine; Ross, Susan R.

In: Journal of Biological Chemistry, Vol. 281, No. 15, 14.04.2006, p. 10243-10249.

Research output: Contribution to journalArticle

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