Identification of UDP-glucuronosyltransferase 1A10 in non-malignant and malignant human breast tissues

Athena Davenport, Beverly Lyn-Cook, Anna Radominska-Pandya

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

UGT1A10 was recently identified as the major isoform that conjugates estrogens. In this study, real-time PCR revealed high levels of UGT1A10 and UGT2B7 mRNA in human breast tissues. The expression of UGT1A10 in breast was a novel finding. UGT1A10 and UGT2B7 mRNAs were differentially expressed among normal and malignant specimens. Their overall expression was significantly decreased in breast carcinomas as compared to normal breast specimens (UGT1A10: 68 ± 26 vs. 252 ± 86, respectively; p < 0.05) and (UGT2B7: 1.4 ± 0.7 vs. 12 ± 4, respectively; p < 0.05). Interestingly, in African American women, UGT1A10 expression was significantly decreased in breast carcinomas in comparison to normals (57 ± 35 vs. 397 ± 152, respectively; p < 0.05). Among Caucasian women, UGT2B7 was significantly decreased in breast carcinomas in comparison to normals (1.1 ± 0.5 vs. 13.5 ± 6, respectively; p < 0.05). Glucuronidation of 4-hydroxylated estrone (4-OHE1) was significantly reduced in breast carcinomas compared to normals (30 ± 15 vs. 106 ± 31, respectively; p < 0.05). Differential down-regulation of UGT1A10 and UGT2B7 mRNAs, protein, and activity in breast carcinomas compared to the adjacent normal breast specimens from the same donor were also found. These data illustrate the novel finding of UGT1A10 in human breast and confirm the expression of UGT2B7. Significant individual variation and down-regulation of expression in breast carcinomas of both isoforms were also demonstrated. These findings provide evidence that decreased UGT expression and activity could result in the promotion of carcinogenesis.

Original languageEnglish (US)
Pages (from-to)611-620
Number of pages10
JournalSteroids
Volume73
Issue number6
DOIs
StatePublished - Jan 1 2008

Fingerprint

Glucuronosyltransferase
Breast
Tissue
Breast Neoplasms
Messenger RNA
Protein Isoforms
Down-Regulation
bilirubin uridine-diphosphoglucuronosyl transferase 1A10
Estrone
African Americans
Real-Time Polymerase Chain Reaction
Estrogens
Carcinogenesis
Tissue Donors

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Identification of UDP-glucuronosyltransferase 1A10 in non-malignant and malignant human breast tissues. / Davenport, Athena; Lyn-Cook, Beverly; Radominska-Pandya, Anna.

In: Steroids, Vol. 73, No. 6, 01.01.2008, p. 611-620.

Research output: Contribution to journalArticle

Davenport, Athena ; Lyn-Cook, Beverly ; Radominska-Pandya, Anna. / Identification of UDP-glucuronosyltransferase 1A10 in non-malignant and malignant human breast tissues. In: Steroids. 2008 ; Vol. 73, No. 6. pp. 611-620.
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