Ig H and L chain contributions to autoimmune specificities

Marko Radic, M. A. Mascelli, J. Erikson, H. Shan, M. Weigert

Research output: Contribution to journalArticle

206 Citations (Scopus)

Abstract

An Ig H chain expression vector has been constructed by using the V region of 3H9, an antibody that binds ssDNA, dsDNA, and cardiolipin. The H chain construct was transfected into six hybridoma cell lines expressing Ig L chains. All resulting H and L chain combinations had at least some affinity for ssDNA, whereas five also bound dsDNA to a similar degree as 3H9. The loss of dsDNA binding was correlated with a single amino acid difference between two V(κ)8 L chains. A further characteristic of 3H9, its immunofluorescent staining pattern, was shared by four of the recombinant antibodies, whereas its specificity for cardiolipin was shared with five. The transfections reported here show that a V(κ)3 L chain confers specificity for an RNA-associated epitope and that a V(κ)21E L chain prevents cardiolipin binding. These experiments suggest that the 3H9H chain contributes essential determinants required for binding to DNA as well as cardiolipin but that L chains can modulate or prevent this binding. L chains may also expand the specificity of a recombinant antibody.

Original languageEnglish (US)
Pages (from-to)176-182
Number of pages7
JournalJournal of Immunology
Volume146
Issue number1
StatePublished - Jan 1 1991

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Cardiolipins
Antibodies
Hybridomas
Transfection
Epitopes
RNA
Staining and Labeling
Amino Acids
Cell Line
DNA

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Radic, M., Mascelli, M. A., Erikson, J., Shan, H., & Weigert, M. (1991). Ig H and L chain contributions to autoimmune specificities. Journal of Immunology, 146(1), 176-182.

Ig H and L chain contributions to autoimmune specificities. / Radic, Marko; Mascelli, M. A.; Erikson, J.; Shan, H.; Weigert, M.

In: Journal of Immunology, Vol. 146, No. 1, 01.01.1991, p. 176-182.

Research output: Contribution to journalArticle

Radic, M, Mascelli, MA, Erikson, J, Shan, H & Weigert, M 1991, 'Ig H and L chain contributions to autoimmune specificities', Journal of Immunology, vol. 146, no. 1, pp. 176-182.
Radic M, Mascelli MA, Erikson J, Shan H, Weigert M. Ig H and L chain contributions to autoimmune specificities. Journal of Immunology. 1991 Jan 1;146(1):176-182.
Radic, Marko ; Mascelli, M. A. ; Erikson, J. ; Shan, H. ; Weigert, M. / Ig H and L chain contributions to autoimmune specificities. In: Journal of Immunology. 1991 ; Vol. 146, No. 1. pp. 176-182.
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