IL-12-assisted immunization against Listeria monocytogenes using replication-restricted VSV-based vectors

Sheri D. Klas, Christy L. Lavine, Michael Whitt, Mark Miller

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Co-administration of IL-12 with vaccine immunogens has proven to be an effective strategy for eliciting potent Th1-biased immunity. Unfortunately, the use of IL-12 as a vaccine component has been limited because it is unstable at ambient temperatures, expensive to produce, and toxic when administered at excessive dosages. Using reverse genetics, we created a recombinant replication-restricted vesicular stomatitis virus that expresses large quantities of an IL-12 fusion protein (VSVΔG-IL12F), but can only establish a single round of infection because the genome does not encode the viral glycoprotein (G protein) that is required for viral entry into host cells. Here, we report that immunization of mice with a poorly immunogenic listerial antigen preparation (LMAg) in combination with VSVΔG-IL12F elicits potent T cell- and B cell-mediated responses that confer protective listerial immunity.

Original languageEnglish (US)
Pages (from-to)1451-1461
Number of pages11
JournalVaccine
Volume24
Issue number9
DOIs
StatePublished - Feb 27 2006

Fingerprint

interleukin-12
Listeria monocytogenes
Interleukin-12
Immunization
immunization
Immunity
Vaccines
immunity
vaccines
antigens
Vesiculovirus
Reverse Genetics
Vesicular Stomatitis
Poisons
B-lymphocytes
glycoproteins
ambient temperature
Glycoproteins
B-Lymphocytes
T-lymphocytes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

IL-12-assisted immunization against Listeria monocytogenes using replication-restricted VSV-based vectors. / Klas, Sheri D.; Lavine, Christy L.; Whitt, Michael; Miller, Mark.

In: Vaccine, Vol. 24, No. 9, 27.02.2006, p. 1451-1461.

Research output: Contribution to journalArticle

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