IL-12-assisted immunization generates CD4+ T cell-mediated immunity to Listeria monocytogenes

Mark Miller, Marianne J. Skeen, Christy L. Lavine, H. Kirk Ziegler

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Mice infected with virulent Listeria monocytogenes develop long-lived acquired immunity. We previously reported that acquired immunity to Listeria could also be elicited by immunizing mice with non-viable Listeria or listerial proteins/peptides in combination with IL-12. Here we show that this IL-12-assisted immunization strategy was effective in class I but not in class II MHC-deficient mice, suggesting that antigen-specific CD4+ T cells are selectively generated using this adjuvant system. We have also evaluated the importance of endogenous production of IFN-γ and IL-12 for the efficacy of IL-12-assisted immunization. IFN-γ-deficient mice immunized with HKLM and IL-12 failed to produce effective Listeria-specific responses. In contrast, IL-12-deficient mice were able to generate protective antigen-specific T cell responses in response to immunization with HKLM and IL-12, indicating that exogenous IL-12 is sufficient to initiate a cytokine cascade that results in a potent TH1 response. IL-12-assisted immunization provides a model in which both the generation and effector mechanisms of anti-bacterial antigen-specific CD4+ effector cells can be analyzed.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalCellular Immunology
Volume222
Issue number1
DOIs
StatePublished - Jan 1 2003

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Listeria monocytogenes
Interleukin-12
Cellular Immunity
Immunization
T-Lymphocytes
Listeria
Adaptive Immunity
Bacterial Antigens
CD4 Antigens
Cytokines
Antigens
Peptides

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

IL-12-assisted immunization generates CD4+ T cell-mediated immunity to Listeria monocytogenes. / Miller, Mark; Skeen, Marianne J.; Lavine, Christy L.; Ziegler, H. Kirk.

In: Cellular Immunology, Vol. 222, No. 1, 01.01.2003, p. 1-14.

Research output: Contribution to journalArticle

Miller, Mark ; Skeen, Marianne J. ; Lavine, Christy L. ; Ziegler, H. Kirk. / IL-12-assisted immunization generates CD4+ T cell-mediated immunity to Listeria monocytogenes. In: Cellular Immunology. 2003 ; Vol. 222, No. 1. pp. 1-14.
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