IL-12 receptor expression on t lymphocyte subsets during the development of an immune response to listeria

Marianne J. Skeen, Mark Miller, H. Kirk Ziegler

Research output: Contribution to journalArticle

Abstract

Expression of IL-12 receptors (R) on T cell subsets was examined by twocolor flow cytometry. Cells were incubated with saturating amounts of r IL-12, followed by a biotinylated anti-U-12 mAb, and finally by streptavidin-PE and a FITC-conjugated T cell subset marker. D-12R expression was higher on peritoneal 7 T cells than on a T cells in naive mice as indicated by both the percentage of cells which were receptor-positive and the mean fluorescence intensity. Only modest upregulation of receptor expression was seen on a T cells after i.p. infection with Listeria or injection of inflammatory stimuli. In contrast, IL-12R expression increased within 24 hrs on 7 T cells and remained elevated in Listeria-immune mice. Within the a T cell population, IL-12R expression was greater on CD4+ cells than on CD8+ cells. IL-12R expression on NK cells, B cells and granulocytes is under investigation. The data suggest that 76 T cells are poised to respond early to the increased amounts of IL-12 which are produced by macrophages after infection or inflammation. We have previously reported that purified 7 T cells produce IFN 7 in response to IL12 in combination with IL-i. This technique provides an additional tool for examining the relative contributions of lymphoid subsets to host defense.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996
Externally publishedYes

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Interleukin-12 Receptors
Listeria
T-cells
Lymphocytes
interleukin-12
Lymphocyte Subsets
lymphocytes
T-lymphocytes
immune response
T-Lymphocytes
receptors
Interleukin-12
T-Lymphocyte Subsets
Listeriosis
Streptavidin
Fluorescein-5-isothiocyanate
cells
streptavidin
Granulocytes
Natural Killer Cells

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

IL-12 receptor expression on t lymphocyte subsets during the development of an immune response to listeria. / Skeen, Marianne J.; Miller, Mark; Kirk Ziegler, H.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

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