Imaging of Boron in Tissue at the Cellular Level for Boron Neutron Capture Therapy

H. F. Arlinghaus, M. T. Spaar, R. C. Switzer, George Kabalka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glioblastoma multiforme, and other tumors involving the brain, are undergoing experimental treatment with a promising new technique: boron neutron capture therapy (BNCT). BNCT relies on the capture of thermal neutrons by boron deposited biochemically in the tumor and the subsequent fission of the boron into energetic lithium ions and a particles. An important requirement for improved BNCT is the development of more selective boron delivery mechanisms. The ability to image the boron concentration in tissue sections and even inside individual cells would be an important aid in the development of these delivery mechanisms. We have compared both sputter-initiated resonance ionization microprobe (SIRIMP), which combines resonance ionization with a high-energy pulsed focused sputter ion beam and mass spectrometric detection of ions, with laser atomization resonance ionization microprobe (LARIMP), which uses a laser pulse instead of an ion pulse for the atomization process, to determine their characteristics in locating and quantifying boron concentrations as a function of position in tissues obtained from a rat which had been infused with a BNCT drug. The data show that the SIRIMP/LARIMP techniques are well suited for quantitative and ultrasensitive imaging of boron trace element concentrations in biological tissue sections. The LARIMP mode could be used to quickly determine the spatial boron concentration with intercellular resolution over large areas down to the low nanograms-per-gram level, while the SIRIMP mode could be used to determine the spatial boron concentration and its variability in intracellular areas.

Original languageEnglish (US)
Pages (from-to)3169-3176
Number of pages8
JournalAnalytical Chemistry
Volume69
Issue number16
DOIs
StatePublished - Aug 15 1997
Externally publishedYes

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Boron
Neutrons
Tissue
Imaging techniques
Ionization
Atomization
Ions
Lasers
Tumors
Laser pulses
Drug therapy
Trace Elements
Laser modes
Lithium
Ion beams
Rats
Brain

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Imaging of Boron in Tissue at the Cellular Level for Boron Neutron Capture Therapy. / Arlinghaus, H. F.; Spaar, M. T.; Switzer, R. C.; Kabalka, George.

In: Analytical Chemistry, Vol. 69, No. 16, 15.08.1997, p. 3169-3176.

Research output: Contribution to journalArticle

Arlinghaus, H. F. ; Spaar, M. T. ; Switzer, R. C. ; Kabalka, George. / Imaging of Boron in Tissue at the Cellular Level for Boron Neutron Capture Therapy. In: Analytical Chemistry. 1997 ; Vol. 69, No. 16. pp. 3169-3176.
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