Immunity to type XI collagen in mice

Evidence that the α3(XI) chain of type XI collagen and the α1(II) chain of type II collagen share arthritogenic determinants and induce arthritis in DBA/1 mice

M. A. Cremer, K. Terato, J. M. Seyer, W. C. Watson, G. O. O'Hagan, A. S. Townes, Andrew Kang

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Abstract

To determine whether native bovine type XI collagen (BXI) is arthritogenic, five strains of inbred mice were immunized with BXI/CFA. Arthritis was not observed in any of these strains, though it was prevalent in DBA/1 and B10.RIII controls immunized with bovine type II collagen (BII). Antisera from BXI-immunized mice reacted with mouse type XI collagen (MsXI), weakly with the α-chains of BXI, and minimally with mouse type II collagen (MsII). However, antisera to BII reacted with MsII and MsXI, indicating antibodies to conformation-independent epitopes shared by α1(II) and α3(XI). Mice immunized with BXI containing a small amount of BII developed arthritis much like those immunized with BII; sera from these mice reacted with MsXI and MsII. Delayed-type hypersensitivity responses differed from IgG responses, i.e., BXI elicited responses to α1(XI), α2(XI), α3(XI), and α1(II); BII, to α3(XI) and α1(II) exclusively. To determine whether α1(XI), α2(XI), α3(XI), and α1(II) are arthritogenic, DBA/1J mice were immunized with each α-chain. Arthritis was seen in mice injected with α3(XI) or α1(II). Sera to both α-chains reacted similarly with MsII and peptide fragment α1(II)-CB11. Epitope mapping using polyclonal and mAb to type II collagen revealed that all polyclonal and 11 of 14 mAb reacted with α3(XI) and α1(II), whereas three mAb reacted only with α1(II). In conclusion, BXI is immunogenic but not arthritogenic in five strains of mice, whereas α3(XI) and α1(II) are arthritogenic and immunogenic in DBA/1 mice and share ≥11 epitopes recognized by autoantibody.

Original languageEnglish (US)
Pages (from-to)4130-4137
Number of pages8
JournalJournal of Immunology
Volume146
Issue number12
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Collagen Type XI
Inbred DBA Mouse
Collagen Type II
Collagen Type I
Arthritis
Immunity
Immune Sera
Epitopes
Epitope Mapping
Inbred Strains Mice
Peptide Fragments
Delayed Hypersensitivity
Serum
Autoantibodies
Immunoglobulin G
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Immunity to type XI collagen in mice : Evidence that the α3(XI) chain of type XI collagen and the α1(II) chain of type II collagen share arthritogenic determinants and induce arthritis in DBA/1 mice. / Cremer, M. A.; Terato, K.; Seyer, J. M.; Watson, W. C.; O'Hagan, G. O.; Townes, A. S.; Kang, Andrew.

In: Journal of Immunology, Vol. 146, No. 12, 01.01.1991, p. 4130-4137.

Research output: Contribution to journalArticle

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title = "Immunity to type XI collagen in mice: Evidence that the α3(XI) chain of type XI collagen and the α1(II) chain of type II collagen share arthritogenic determinants and induce arthritis in DBA/1 mice",
abstract = "To determine whether native bovine type XI collagen (BXI) is arthritogenic, five strains of inbred mice were immunized with BXI/CFA. Arthritis was not observed in any of these strains, though it was prevalent in DBA/1 and B10.RIII controls immunized with bovine type II collagen (BII). Antisera from BXI-immunized mice reacted with mouse type XI collagen (MsXI), weakly with the α-chains of BXI, and minimally with mouse type II collagen (MsII). However, antisera to BII reacted with MsII and MsXI, indicating antibodies to conformation-independent epitopes shared by α1(II) and α3(XI). Mice immunized with BXI containing a small amount of BII developed arthritis much like those immunized with BII; sera from these mice reacted with MsXI and MsII. Delayed-type hypersensitivity responses differed from IgG responses, i.e., BXI elicited responses to α1(XI), α2(XI), α3(XI), and α1(II); BII, to α3(XI) and α1(II) exclusively. To determine whether α1(XI), α2(XI), α3(XI), and α1(II) are arthritogenic, DBA/1J mice were immunized with each α-chain. Arthritis was seen in mice injected with α3(XI) or α1(II). Sera to both α-chains reacted similarly with MsII and peptide fragment α1(II)-CB11. Epitope mapping using polyclonal and mAb to type II collagen revealed that all polyclonal and 11 of 14 mAb reacted with α3(XI) and α1(II), whereas three mAb reacted only with α1(II). In conclusion, BXI is immunogenic but not arthritogenic in five strains of mice, whereas α3(XI) and α1(II) are arthritogenic and immunogenic in DBA/1 mice and share ≥11 epitopes recognized by autoantibody.",
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T2 - Evidence that the α3(XI) chain of type XI collagen and the α1(II) chain of type II collagen share arthritogenic determinants and induce arthritis in DBA/1 mice

AU - Cremer, M. A.

AU - Terato, K.

AU - Seyer, J. M.

AU - Watson, W. C.

AU - O'Hagan, G. O.

AU - Townes, A. S.

AU - Kang, Andrew

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N2 - To determine whether native bovine type XI collagen (BXI) is arthritogenic, five strains of inbred mice were immunized with BXI/CFA. Arthritis was not observed in any of these strains, though it was prevalent in DBA/1 and B10.RIII controls immunized with bovine type II collagen (BII). Antisera from BXI-immunized mice reacted with mouse type XI collagen (MsXI), weakly with the α-chains of BXI, and minimally with mouse type II collagen (MsII). However, antisera to BII reacted with MsII and MsXI, indicating antibodies to conformation-independent epitopes shared by α1(II) and α3(XI). Mice immunized with BXI containing a small amount of BII developed arthritis much like those immunized with BII; sera from these mice reacted with MsXI and MsII. Delayed-type hypersensitivity responses differed from IgG responses, i.e., BXI elicited responses to α1(XI), α2(XI), α3(XI), and α1(II); BII, to α3(XI) and α1(II) exclusively. To determine whether α1(XI), α2(XI), α3(XI), and α1(II) are arthritogenic, DBA/1J mice were immunized with each α-chain. Arthritis was seen in mice injected with α3(XI) or α1(II). Sera to both α-chains reacted similarly with MsII and peptide fragment α1(II)-CB11. Epitope mapping using polyclonal and mAb to type II collagen revealed that all polyclonal and 11 of 14 mAb reacted with α3(XI) and α1(II), whereas three mAb reacted only with α1(II). In conclusion, BXI is immunogenic but not arthritogenic in five strains of mice, whereas α3(XI) and α1(II) are arthritogenic and immunogenic in DBA/1 mice and share ≥11 epitopes recognized by autoantibody.

AB - To determine whether native bovine type XI collagen (BXI) is arthritogenic, five strains of inbred mice were immunized with BXI/CFA. Arthritis was not observed in any of these strains, though it was prevalent in DBA/1 and B10.RIII controls immunized with bovine type II collagen (BII). Antisera from BXI-immunized mice reacted with mouse type XI collagen (MsXI), weakly with the α-chains of BXI, and minimally with mouse type II collagen (MsII). However, antisera to BII reacted with MsII and MsXI, indicating antibodies to conformation-independent epitopes shared by α1(II) and α3(XI). Mice immunized with BXI containing a small amount of BII developed arthritis much like those immunized with BII; sera from these mice reacted with MsXI and MsII. Delayed-type hypersensitivity responses differed from IgG responses, i.e., BXI elicited responses to α1(XI), α2(XI), α3(XI), and α1(II); BII, to α3(XI) and α1(II) exclusively. To determine whether α1(XI), α2(XI), α3(XI), and α1(II) are arthritogenic, DBA/1J mice were immunized with each α-chain. Arthritis was seen in mice injected with α3(XI) or α1(II). Sera to both α-chains reacted similarly with MsII and peptide fragment α1(II)-CB11. Epitope mapping using polyclonal and mAb to type II collagen revealed that all polyclonal and 11 of 14 mAb reacted with α3(XI) and α1(II), whereas three mAb reacted only with α1(II). In conclusion, BXI is immunogenic but not arthritogenic in five strains of mice, whereas α3(XI) and α1(II) are arthritogenic and immunogenic in DBA/1 mice and share ≥11 epitopes recognized by autoantibody.

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