Immunochemical classification of nonisolated light chains of immunoglobulins

C. L. McLaughlin, Alan Solomon

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Antigenic sites of the light chain are often masked in the intact immunoglobulin (Ig) molecule; and because many of the anti light chain antisera have stronger reactivity with the free light chain than with the intact Ig molecule, a microtechnique for reduction and alkylation of homogeneous immunoglobulins which does not require isolation of the light chain before immunochemical typing was used in preparation of type K myeloma proteins and M macroglobulins for immunochemical analyses without group specific antisera. Antisera specific for kappa I, kappa II, kappa III, and kappa IV light chains were used in immunodiffusion analyses of the reduced and alkylated Ig; antigenic identity between the treated Ig and homologous isolated light chains readily demonstrable. The classification of the light chain of proteins of known antibody activity, e.g., cold agglutinins, as well as the study of proteins available in small quantity, was facilitated by the use of the microtechnique for reduction and alkylation.

Original languageEnglish (US)
Pages (from-to)1369-1372
Number of pages4
JournalJournal of Immunology
Volume113
Issue number4
StatePublished - Dec 1 1974

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Immunoglobulin Light Chains
Light
Immunoglobulins
Immune Sera
Alkylation
Macroglobulins
Immunodiffusion
Proteins
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Immunochemical classification of nonisolated light chains of immunoglobulins. / McLaughlin, C. L.; Solomon, Alan.

In: Journal of Immunology, Vol. 113, No. 4, 01.12.1974, p. 1369-1372.

Research output: Contribution to journalArticle

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