Immunocytochemical analysis of tumor cells in pre- and post-culture peripheral blood progenitor cell collections from breast cancer patients

A. A. Ross, M. Loudovaris, B. Hazelton, C. H. Weaver, Lee Schwartzberg, J. G. Bender

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We examined peripheral blood progenitor cell (PBPC) collections and CD34+-selected fractions cultured in PIXY321, a fusion protein comprising analog interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) domains, for the presence of contaminating tumor cells from 14 patients with advanced-stage breast cancer. Five of the 14 (36%) pre-culture PBPC specimens contained immunocytochemically (ICC)-detectable tumor cells using two different cocktails of monoclonal antibodies (mAbs). After 10 days in culture with PIXY321, the CD34+-selected fractions showed a median 23.6-fold expansion of hematopoietic cells. No ICC-positive tumor cells were detected in any post-culture specimens. We conclude that in vitro expansion of CD34+-selected PBPCs with PIXY321 can expand hematopoietic cell populations apparently without risk of expanding contaminating breast cancer cell populations.

Original languageEnglish (US)
Pages (from-to)1478-1483
Number of pages6
JournalExperimental Hematology
Volume23
Issue number14
StatePublished - Dec 1 1995
Externally publishedYes

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Blood Cells
Stem Cells
Breast Neoplasms
Neoplasms
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Population
Monoclonal Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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Immunocytochemical analysis of tumor cells in pre- and post-culture peripheral blood progenitor cell collections from breast cancer patients. / Ross, A. A.; Loudovaris, M.; Hazelton, B.; Weaver, C. H.; Schwartzberg, Lee; Bender, J. G.

In: Experimental Hematology, Vol. 23, No. 14, 01.12.1995, p. 1478-1483.

Research output: Contribution to journalArticle

Ross, A. A. ; Loudovaris, M. ; Hazelton, B. ; Weaver, C. H. ; Schwartzberg, Lee ; Bender, J. G. / Immunocytochemical analysis of tumor cells in pre- and post-culture peripheral blood progenitor cell collections from breast cancer patients. In: Experimental Hematology. 1995 ; Vol. 23, No. 14. pp. 1478-1483.
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