Immunocytochemical studies of cathepsin D in human skeletal muscle

John N. Whitaker, Tulio Bertorini, Jerry R. Mendell

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The distribution of cathepsin D, an acidic endopeptidase, was localized by immunocytochemistry in human skeletal muscle obtained from 34 persons with a variety of neuromuscular disorders. Normal human skeletal muscle contained small amounts of cathepsin D, all of which was found close to the sarcolemmal membrane. Immunoreative cathepsin D was present in the cytoplasm of many infiltrating phagocytic cells and was increased in skeletal muscle fibers from patients with muscular dystrophies, inflammatory myopathies, rhabdomyolysis, acid maltase deficiency, and neurogenic atrophy. In cases of Duchenne type muscular dystrophy, the increase in cathepsin D was especially prominent in small regenerating fibers, in which it was visualized at the ultrastructural level in lysosome‐like organelles anextralysosomal locations. The function of cathepsin D in skeletal muscle is unclear, but the present findings suggest a possible role in muscle regeneration and repair. Such a role would necessitate careful selection of drugs which interfere with proteolytic activity if they are to be used as therapeutic agents in treating neuromuscular diseases.

Original languageEnglish (US)
Pages (from-to)133-142
Number of pages10
JournalAnnals of Neurology
Volume13
Issue number2
DOIs
StatePublished - Jan 1 1983

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Cathepsin D
Skeletal Muscle
Glycogen Storage Disease Type II
Endopeptidases
Neuromuscular Diseases
Rhabdomyolysis
Myositis
Duchenne Muscular Dystrophy
Muscular Dystrophies
Skeletal Muscle Fibers
Phagocytes
Organelles
Atrophy
Regeneration
Cytoplasm
Immunohistochemistry
Muscles
Membranes
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Immunocytochemical studies of cathepsin D in human skeletal muscle. / Whitaker, John N.; Bertorini, Tulio; Mendell, Jerry R.

In: Annals of Neurology, Vol. 13, No. 2, 01.01.1983, p. 133-142.

Research output: Contribution to journalArticle

Whitaker, John N. ; Bertorini, Tulio ; Mendell, Jerry R. / Immunocytochemical studies of cathepsin D in human skeletal muscle. In: Annals of Neurology. 1983 ; Vol. 13, No. 2. pp. 133-142.
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