Immunogenic and antigenic epitopes of Ig. XXV. Monoclonal antibodies that differentiate the Mcg+/Mcg- and Oz+/Oz- C region isotypes of human λ L chains

M. R. Walker, Alan Solomon, D. T. Weiss, H. F. Deutch, R. Jefferis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The C region of human λ L chains is specified by multiple C(λ) genes of which three - C(λ)1, C(λ)2, and C(λ)3 - encode for the isotypes designated Mcg+, Kern- Oz-, and Kern- Oz+, respectively. The Mcg, Kern, and Oz factors have been characterized by sequence differences involving specific C(λ) amino acid residues. They have also been recognized serologically by polyclonal antisera but, with rare exception, these reagents are no longer available. We have obtained two murine antihuman λ-chain mAb, 14G1 and 14D1, that recognize antigenic determinants specific for the C(λ) isotypes Mcg and Oz, respectively. These antisera have been used to classify as Mcg+/Mcg- or Oz+/Oz- monoclonal λ-chains (Bence Jones proteins) and intact Igλ proteins. There was complete concordance between the chemical and serologic assignment of λ-chains as Mcg+/Mcg- or as Oz+/Oz-; no single protein expressed both isotypes. There was no evident association between the C region isotype Mcg or Oz and the V region subgroup of the protein tested. However, our finding that four of seven amyloid-associated λVI Bence Jones proteins were Oz+ suggests a predominant expression of the C(λ)3 gene product among proteins of this uncommon V(λ) subgroup.

Original languageEnglish (US)
Pages (from-to)1600-1604
Number of pages5
JournalJournal of Immunology
Volume140
Issue number5
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Epitopes
Monoclonal Antibodies
Bence Jones Protein
Immune Sera
Proteins
Amyloid
Amino Acids
Genes

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Immunogenic and antigenic epitopes of Ig. XXV. Monoclonal antibodies that differentiate the Mcg+/Mcg- and Oz+/Oz- C region isotypes of human λ L chains. / Walker, M. R.; Solomon, Alan; Weiss, D. T.; Deutch, H. F.; Jefferis, R.

In: Journal of Immunology, Vol. 140, No. 5, 01.01.1988, p. 1600-1604.

Research output: Contribution to journalArticle

@article{720cc41828ab49c69cc4f1034dbd3fdc,
title = "Immunogenic and antigenic epitopes of Ig. XXV. Monoclonal antibodies that differentiate the Mcg+/Mcg- and Oz+/Oz- C region isotypes of human λ L chains",
abstract = "The C region of human λ L chains is specified by multiple C(λ) genes of which three - C(λ)1, C(λ)2, and C(λ)3 - encode for the isotypes designated Mcg+, Kern- Oz-, and Kern- Oz+, respectively. The Mcg, Kern, and Oz factors have been characterized by sequence differences involving specific C(λ) amino acid residues. They have also been recognized serologically by polyclonal antisera but, with rare exception, these reagents are no longer available. We have obtained two murine antihuman λ-chain mAb, 14G1 and 14D1, that recognize antigenic determinants specific for the C(λ) isotypes Mcg and Oz, respectively. These antisera have been used to classify as Mcg+/Mcg- or Oz+/Oz- monoclonal λ-chains (Bence Jones proteins) and intact Igλ proteins. There was complete concordance between the chemical and serologic assignment of λ-chains as Mcg+/Mcg- or as Oz+/Oz-; no single protein expressed both isotypes. There was no evident association between the C region isotype Mcg or Oz and the V region subgroup of the protein tested. However, our finding that four of seven amyloid-associated λVI Bence Jones proteins were Oz+ suggests a predominant expression of the C(λ)3 gene product among proteins of this uncommon V(λ) subgroup.",
author = "Walker, {M. R.} and Alan Solomon and Weiss, {D. T.} and Deutch, {H. F.} and R. Jefferis",
year = "1988",
month = "1",
day = "1",
language = "English (US)",
volume = "140",
pages = "1600--1604",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "5",

}

TY - JOUR

T1 - Immunogenic and antigenic epitopes of Ig. XXV. Monoclonal antibodies that differentiate the Mcg+/Mcg- and Oz+/Oz- C region isotypes of human λ L chains

AU - Walker, M. R.

AU - Solomon, Alan

AU - Weiss, D. T.

AU - Deutch, H. F.

AU - Jefferis, R.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - The C region of human λ L chains is specified by multiple C(λ) genes of which three - C(λ)1, C(λ)2, and C(λ)3 - encode for the isotypes designated Mcg+, Kern- Oz-, and Kern- Oz+, respectively. The Mcg, Kern, and Oz factors have been characterized by sequence differences involving specific C(λ) amino acid residues. They have also been recognized serologically by polyclonal antisera but, with rare exception, these reagents are no longer available. We have obtained two murine antihuman λ-chain mAb, 14G1 and 14D1, that recognize antigenic determinants specific for the C(λ) isotypes Mcg and Oz, respectively. These antisera have been used to classify as Mcg+/Mcg- or Oz+/Oz- monoclonal λ-chains (Bence Jones proteins) and intact Igλ proteins. There was complete concordance between the chemical and serologic assignment of λ-chains as Mcg+/Mcg- or as Oz+/Oz-; no single protein expressed both isotypes. There was no evident association between the C region isotype Mcg or Oz and the V region subgroup of the protein tested. However, our finding that four of seven amyloid-associated λVI Bence Jones proteins were Oz+ suggests a predominant expression of the C(λ)3 gene product among proteins of this uncommon V(λ) subgroup.

AB - The C region of human λ L chains is specified by multiple C(λ) genes of which three - C(λ)1, C(λ)2, and C(λ)3 - encode for the isotypes designated Mcg+, Kern- Oz-, and Kern- Oz+, respectively. The Mcg, Kern, and Oz factors have been characterized by sequence differences involving specific C(λ) amino acid residues. They have also been recognized serologically by polyclonal antisera but, with rare exception, these reagents are no longer available. We have obtained two murine antihuman λ-chain mAb, 14G1 and 14D1, that recognize antigenic determinants specific for the C(λ) isotypes Mcg and Oz, respectively. These antisera have been used to classify as Mcg+/Mcg- or Oz+/Oz- monoclonal λ-chains (Bence Jones proteins) and intact Igλ proteins. There was complete concordance between the chemical and serologic assignment of λ-chains as Mcg+/Mcg- or as Oz+/Oz-; no single protein expressed both isotypes. There was no evident association between the C region isotype Mcg or Oz and the V region subgroup of the protein tested. However, our finding that four of seven amyloid-associated λVI Bence Jones proteins were Oz+ suggests a predominant expression of the C(λ)3 gene product among proteins of this uncommon V(λ) subgroup.

UR - http://www.scopus.com/inward/record.url?scp=0023945893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023945893&partnerID=8YFLogxK

M3 - Article

VL - 140

SP - 1600

EP - 1604

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 5

ER -