Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years

Veronika Weyerer, Roland Schneckenpointner, Thomas Filbeck, Maximilian Burger, Ferdinand Hofstaedter, Peter J. Wild, Samson W. Fine, Peter A. Humphrey, Louis P. Dehner, Mahul Amin, Josef Rüschoff, Carsten Boltze, Andrea Tannapfel, Ellen Zwarthoff, Antonio Lopez-Beltran, Rodolfo Montironi, Cord Langner, Robert Stoehr, Arndt Hartmann, Johannes Giedl

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Abstract

Bladder tumours in early-onset patients are rare and seem to exhibit unique clinicopathological features. Only few studies have investigated somatic alterations in this specific age of onset group and evidence is accumulating of a distinct molecular behaviour of early-onset bladder tumours. We collected the largest cohort of early-onset tumours of patients 45 years old or younger and aimed to test genomic alterations typically found in bladder cancer. Tumours of 118 early-onset patients were compared with a consecutive group of 113 cases. Immunohistochemistry of TP53, CK20 and Ki-67 was carried out. Molecular analysis was conducted to test for loss of heterozygosity of chromosome 9 and 17, as well as TP53 and FGFR3 mutations. Fisheŕs exact and chi-squared test were appropriately used. No differences in grade/stage characteristics were observed. Overexpressed TP53 was differentially distributed between the two groups. TP53 nuclear accumulation was significantly more frequent in early-onset papillomas, PUNLMPs and pTa low-grade tumours compared to the consecutive cohort (p=0.005). Moreover, chromosome 9 deletions (29.5% vs. 44.6%) and FGFR3 mutations (34.5% vs. 63.7%) were less often detected in early-onset patients (p=0.05 and p<0.0001). By comparing the largest cohort of early-onset bladder cancer patients with an unselected group, we demonstrated that the typical molecular features are not independent of age at diagnosis. Our study supports the hypothesis of a distinct biological behaviour in early-onset tumours.

Original languageEnglish (US)
Article number482
Pages (from-to)323-331
Number of pages9
JournalJournal of Cancer
Volume8
Issue number3
DOIs
StatePublished - Jan 1 2017

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Urinary Bladder
Urinary Bladder Neoplasms
Carcinoma
Chromosomes, Human, Pair 9
Neoplasms
Chromosome Deletion
Chromosomes, Human, Pair 17
Mutation
Loss of Heterozygosity
Papilloma
Age of Onset
Fishes
Age Groups
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Oncology

Cite this

Weyerer, V., Schneckenpointner, R., Filbeck, T., Burger, M., Hofstaedter, F., Wild, P. J., ... Giedl, J. (2017). Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years. Journal of Cancer, 8(3), 323-331. [482]. https://doi.org/10.7150/jca.17482

Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years. / Weyerer, Veronika; Schneckenpointner, Roland; Filbeck, Thomas; Burger, Maximilian; Hofstaedter, Ferdinand; Wild, Peter J.; Fine, Samson W.; Humphrey, Peter A.; Dehner, Louis P.; Amin, Mahul; Rüschoff, Josef; Boltze, Carsten; Tannapfel, Andrea; Zwarthoff, Ellen; Lopez-Beltran, Antonio; Montironi, Rodolfo; Langner, Cord; Stoehr, Robert; Hartmann, Arndt; Giedl, Johannes.

In: Journal of Cancer, Vol. 8, No. 3, 482, 01.01.2017, p. 323-331.

Research output: Contribution to journalArticle

Weyerer, V, Schneckenpointner, R, Filbeck, T, Burger, M, Hofstaedter, F, Wild, PJ, Fine, SW, Humphrey, PA, Dehner, LP, Amin, M, Rüschoff, J, Boltze, C, Tannapfel, A, Zwarthoff, E, Lopez-Beltran, A, Montironi, R, Langner, C, Stoehr, R, Hartmann, A & Giedl, J 2017, 'Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years', Journal of Cancer, vol. 8, no. 3, 482, pp. 323-331. https://doi.org/10.7150/jca.17482
Weyerer V, Schneckenpointner R, Filbeck T, Burger M, Hofstaedter F, Wild PJ et al. Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years. Journal of Cancer. 2017 Jan 1;8(3):323-331. 482. https://doi.org/10.7150/jca.17482
Weyerer, Veronika ; Schneckenpointner, Roland ; Filbeck, Thomas ; Burger, Maximilian ; Hofstaedter, Ferdinand ; Wild, Peter J. ; Fine, Samson W. ; Humphrey, Peter A. ; Dehner, Louis P. ; Amin, Mahul ; Rüschoff, Josef ; Boltze, Carsten ; Tannapfel, Andrea ; Zwarthoff, Ellen ; Lopez-Beltran, Antonio ; Montironi, Rodolfo ; Langner, Cord ; Stoehr, Robert ; Hartmann, Arndt ; Giedl, Johannes. / Immunohistochemical and molecular characterizations in urothelial carcinoma of bladder in patients less than 45 years. In: Journal of Cancer. 2017 ; Vol. 8, No. 3. pp. 323-331.
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