Immunohistochemical localization of AMPA-type glutamate receptor subunits in the nucleus of the Edinger-Westphal in embryonic chick

Claudio A.B. Toledo, Anton Reiner, Reena S. Patel, Adriane W. Vitale, Jordan M. Klein, Bob J. Dalsania, Malinda E.C. Fitzgerald

Research output: Contribution to journalArticle

Abstract

The Edinger-Westphal nucleus (EW) in birds is responsible for the control of pupil constriction, accommodation, and choroidal blood flow. The activation of EW neurons is mediated by the neurotransmitter glutamate, in large part through AMPA-type glutamate receptors (GluRs), whose behavior varies according to the subunit composition. We investigated the developmental expression of the GluR subunits in EW of the chick (Gallus gallus) using immunohistochemistry on tissue from embryonic days 10 through 20 (E10-E20). Of the three antibodies used, one recognized the GluR1 subunit, another the GluR4 subunit, and the third recognized a sequence common to GluR2 and GluR3 subunits. No immunolabeling of EW neurons for any GluR subunits was observed prior to E12, although immunolabeling was seen in somatic oculomotor prior to E12. At E12, immunoreactivity for each of the three antibodies was in only approximately 2% of EW neurons. By E14, the abundance of GluR1+ perikarya in EW had increased to 13%, and for GluR2/3 had increased to 48%. The perikaryal abundance of the immunoreactivity for GluR1 and GluR2/3 declined to 3% and 23%, respectively, by E16. At E14, 33% of EW neurons immunolabeled for GluR4, and their frequency increased to 43% by E16, and remained at that approximate percentage through hatching. The increased expression of GluR1 and GluR4 in EW at E14 coincides with the reported onset of the expression of the calcium-binding protein parvalbumin, and the calcium currents associated with AMPA receptors formed by these two subunits may play a role in the occurrence of parvalbumin expression.

Original languageEnglish (US)
Pages (from-to)199-203
Number of pages5
JournalNeuroscience Letters
Volume498
Issue number3
DOIs
StatePublished - Jul 12 2011

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AMPA Receptors
Glutamate Receptors
Neurons
Parvalbumins
Calcium-Binding Proteins
Edinger-Westphal Nucleus
Antibodies
Pupil
Constriction
Birds
Neurotransmitter Agents
Glutamic Acid
Chickens
Immunohistochemistry
Calcium

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Immunohistochemical localization of AMPA-type glutamate receptor subunits in the nucleus of the Edinger-Westphal in embryonic chick. / Toledo, Claudio A.B.; Reiner, Anton; Patel, Reena S.; Vitale, Adriane W.; Klein, Jordan M.; Dalsania, Bob J.; Fitzgerald, Malinda E.C.

In: Neuroscience Letters, Vol. 498, No. 3, 12.07.2011, p. 199-203.

Research output: Contribution to journalArticle

Toledo, Claudio A.B. ; Reiner, Anton ; Patel, Reena S. ; Vitale, Adriane W. ; Klein, Jordan M. ; Dalsania, Bob J. ; Fitzgerald, Malinda E.C. / Immunohistochemical localization of AMPA-type glutamate receptor subunits in the nucleus of the Edinger-Westphal in embryonic chick. In: Neuroscience Letters. 2011 ; Vol. 498, No. 3. pp. 199-203.
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abstract = "The Edinger-Westphal nucleus (EW) in birds is responsible for the control of pupil constriction, accommodation, and choroidal blood flow. The activation of EW neurons is mediated by the neurotransmitter glutamate, in large part through AMPA-type glutamate receptors (GluRs), whose behavior varies according to the subunit composition. We investigated the developmental expression of the GluR subunits in EW of the chick (Gallus gallus) using immunohistochemistry on tissue from embryonic days 10 through 20 (E10-E20). Of the three antibodies used, one recognized the GluR1 subunit, another the GluR4 subunit, and the third recognized a sequence common to GluR2 and GluR3 subunits. No immunolabeling of EW neurons for any GluR subunits was observed prior to E12, although immunolabeling was seen in somatic oculomotor prior to E12. At E12, immunoreactivity for each of the three antibodies was in only approximately 2{\%} of EW neurons. By E14, the abundance of GluR1+ perikarya in EW had increased to 13{\%}, and for GluR2/3 had increased to 48{\%}. The perikaryal abundance of the immunoreactivity for GluR1 and GluR2/3 declined to 3{\%} and 23{\%}, respectively, by E16. At E14, 33{\%} of EW neurons immunolabeled for GluR4, and their frequency increased to 43{\%} by E16, and remained at that approximate percentage through hatching. The increased expression of GluR1 and GluR4 in EW at E14 coincides with the reported onset of the expression of the calcium-binding protein parvalbumin, and the calcium currents associated with AMPA receptors formed by these two subunits may play a role in the occurrence of parvalbumin expression.",
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AU - Fitzgerald, Malinda E.C.

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