Immunohistochemical profile of Myogenin and MyoD1 does not support skeletal muscle lineage in alveolar soft part sarcoma

A study of 19 tumors

José A. Gómez, Mahul Amin, Jae Y. Ro, Michael D. Linden, Min W. Lee, Richard J. Zarbo

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Background. - The histogenesis of alveolar soft part sarcoma remains elusive. Myogenic origin is favored, although conflicting data on immunohistochemical demonstration of muscle-associated markers exist. Myogenin and MyoD1, transcription factors of the myogenic determination family, have crucial roles in commitment and differentiation of mesenchymal progenitor cells to myogenic lineage and in maintenance of skeletal muscle phenotype. Their immunohistochemical detection is specific in characterization of rhabdomyosarcoma. Methods. - Antibodies for myogenin, MyoD1, desmin, and muscle-specific actin were employed on a large series of cases (n = 19) of formalin-fixed, paraffin-embedded alveolar soft part sarcoma. Results. - Minimal scattered nuclear staining was seen with myogenin. All cases had pronounced, nonspecific granular cytoplasmic immunostaining with MyoD1; nuclei were negative. All tumors were negative for desmin and muscle-specific actin. Ultrastructural study in 10 cases failed to reveal features of skeletal muscle differentiation. Conclusions. - Cytoplasmic staining with MyoD1 in alveolar soft part sarcoma may correspond to cross-reactivity with an undetermined cytoplasmic antigen. The lack of immunostaining with myogenin, MyoD1, desmin, and muscle-specific actin provides evidence against a myogenic origin for alveolar soft part sarcoma.

Original languageEnglish (US)
Pages (from-to)503-507
Number of pages5
JournalArchives of Pathology and Laboratory Medicine
Volume123
Issue number6
StatePublished - 1999
Externally publishedYes

Fingerprint

Alveolar Soft Part Sarcoma
Myogenin
Desmin
Skeletal Muscle
Actins
Muscles
Neoplasms
Staining and Labeling
Rhabdomyosarcoma
Mesenchymal Stromal Cells
Paraffin
Formaldehyde
Transcription Factors
Maintenance
Phenotype
Antigens
Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Immunohistochemical profile of Myogenin and MyoD1 does not support skeletal muscle lineage in alveolar soft part sarcoma : A study of 19 tumors. / Gómez, José A.; Amin, Mahul; Ro, Jae Y.; Linden, Michael D.; Lee, Min W.; Zarbo, Richard J.

In: Archives of Pathology and Laboratory Medicine, Vol. 123, No. 6, 1999, p. 503-507.

Research output: Contribution to journalArticle

@article{b88f6f813e8d4a5cacdcbb2845eda5c2,
title = "Immunohistochemical profile of Myogenin and MyoD1 does not support skeletal muscle lineage in alveolar soft part sarcoma: A study of 19 tumors",
abstract = "Background. - The histogenesis of alveolar soft part sarcoma remains elusive. Myogenic origin is favored, although conflicting data on immunohistochemical demonstration of muscle-associated markers exist. Myogenin and MyoD1, transcription factors of the myogenic determination family, have crucial roles in commitment and differentiation of mesenchymal progenitor cells to myogenic lineage and in maintenance of skeletal muscle phenotype. Their immunohistochemical detection is specific in characterization of rhabdomyosarcoma. Methods. - Antibodies for myogenin, MyoD1, desmin, and muscle-specific actin were employed on a large series of cases (n = 19) of formalin-fixed, paraffin-embedded alveolar soft part sarcoma. Results. - Minimal scattered nuclear staining was seen with myogenin. All cases had pronounced, nonspecific granular cytoplasmic immunostaining with MyoD1; nuclei were negative. All tumors were negative for desmin and muscle-specific actin. Ultrastructural study in 10 cases failed to reveal features of skeletal muscle differentiation. Conclusions. - Cytoplasmic staining with MyoD1 in alveolar soft part sarcoma may correspond to cross-reactivity with an undetermined cytoplasmic antigen. The lack of immunostaining with myogenin, MyoD1, desmin, and muscle-specific actin provides evidence against a myogenic origin for alveolar soft part sarcoma.",
author = "G{\'o}mez, {Jos{\'e} A.} and Mahul Amin and Ro, {Jae Y.} and Linden, {Michael D.} and Lee, {Min W.} and Zarbo, {Richard J.}",
year = "1999",
language = "English (US)",
volume = "123",
pages = "503--507",
journal = "Archives of Pathology and Laboratory Medicine",
issn = "0003-9985",
publisher = "College of American Pathologists",
number = "6",

}

TY - JOUR

T1 - Immunohistochemical profile of Myogenin and MyoD1 does not support skeletal muscle lineage in alveolar soft part sarcoma

T2 - A study of 19 tumors

AU - Gómez, José A.

AU - Amin, Mahul

AU - Ro, Jae Y.

AU - Linden, Michael D.

AU - Lee, Min W.

AU - Zarbo, Richard J.

PY - 1999

Y1 - 1999

N2 - Background. - The histogenesis of alveolar soft part sarcoma remains elusive. Myogenic origin is favored, although conflicting data on immunohistochemical demonstration of muscle-associated markers exist. Myogenin and MyoD1, transcription factors of the myogenic determination family, have crucial roles in commitment and differentiation of mesenchymal progenitor cells to myogenic lineage and in maintenance of skeletal muscle phenotype. Their immunohistochemical detection is specific in characterization of rhabdomyosarcoma. Methods. - Antibodies for myogenin, MyoD1, desmin, and muscle-specific actin were employed on a large series of cases (n = 19) of formalin-fixed, paraffin-embedded alveolar soft part sarcoma. Results. - Minimal scattered nuclear staining was seen with myogenin. All cases had pronounced, nonspecific granular cytoplasmic immunostaining with MyoD1; nuclei were negative. All tumors were negative for desmin and muscle-specific actin. Ultrastructural study in 10 cases failed to reveal features of skeletal muscle differentiation. Conclusions. - Cytoplasmic staining with MyoD1 in alveolar soft part sarcoma may correspond to cross-reactivity with an undetermined cytoplasmic antigen. The lack of immunostaining with myogenin, MyoD1, desmin, and muscle-specific actin provides evidence against a myogenic origin for alveolar soft part sarcoma.

AB - Background. - The histogenesis of alveolar soft part sarcoma remains elusive. Myogenic origin is favored, although conflicting data on immunohistochemical demonstration of muscle-associated markers exist. Myogenin and MyoD1, transcription factors of the myogenic determination family, have crucial roles in commitment and differentiation of mesenchymal progenitor cells to myogenic lineage and in maintenance of skeletal muscle phenotype. Their immunohistochemical detection is specific in characterization of rhabdomyosarcoma. Methods. - Antibodies for myogenin, MyoD1, desmin, and muscle-specific actin were employed on a large series of cases (n = 19) of formalin-fixed, paraffin-embedded alveolar soft part sarcoma. Results. - Minimal scattered nuclear staining was seen with myogenin. All cases had pronounced, nonspecific granular cytoplasmic immunostaining with MyoD1; nuclei were negative. All tumors were negative for desmin and muscle-specific actin. Ultrastructural study in 10 cases failed to reveal features of skeletal muscle differentiation. Conclusions. - Cytoplasmic staining with MyoD1 in alveolar soft part sarcoma may correspond to cross-reactivity with an undetermined cytoplasmic antigen. The lack of immunostaining with myogenin, MyoD1, desmin, and muscle-specific actin provides evidence against a myogenic origin for alveolar soft part sarcoma.

UR - http://www.scopus.com/inward/record.url?scp=0033018155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033018155&partnerID=8YFLogxK

M3 - Article

VL - 123

SP - 503

EP - 507

JO - Archives of Pathology and Laboratory Medicine

JF - Archives of Pathology and Laboratory Medicine

SN - 0003-9985

IS - 6

ER -