Immunohistochemical Studies of the Adult Human Ovary

Possible Contribution of Immune and Epithelial Factors to Folliculogenesis

ANTONÍN BuKOVSKý, Jeffrey Keenan, Michael Caudle, JAY WIMALASENA, Nirmala Upadhyaya, Stuart Van Meter

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66 Citations (Scopus)

Abstract

PROBLEM: Formation of primordial follicles in adult ovaries could be a cryptic process limited to relatively small areas of the ovarian cortex and occurring during a certain stage of the menstrual cycle. Such an event may require a specific milieu provided by factors involved in developmental processes, i.e., morphoregulatory molecules and macrophages. METHOD: Adult human ovaries were investigated by immunohistochemistry for surface epithelium and granulosa cell markers (cytokeratin 18 and MHC class I), immune system‐related morphoregulatory molecules (Thy‐1 glycoprotein and N‐CAM), and macrophage phenotypes (CD14, CD68, and MHC class II). RESULTS: In some ovaries 300–500 μm areas of surface epithelium were overgrown by tunica albuginea, descended into the stroma, and apparently fragmented into individual small (20–40 μm) follicle‐like cell nests. Differentiation of the surface epithelium was accompanied by macrophages and Thy‐1 glycoprotein. Small segments of surface epithelium showed N‐CAM and a lacked MHC class I expression. In such segments, clear spherical germ‐like cells migrated into the deeper stroma, associated with the microvasculature, and eventually aggregated with follicle‐like cell nests. CONCLUSIONS: Our data suggest that surface epithelium may be involved in the formation of some primordial follicles in adult ovaries. This process, and further follicular fate, may require a precise interplay of immune system related morphoregulatory molecules and macrophages. 1995 Munksgaard

Original languageEnglish (US)
Pages (from-to)323-340
Number of pages18
JournalAmerican Journal of Reproductive Immunology
Volume33
Issue number4
DOIs
StatePublished - Jan 1 1995

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Immunologic Factors
Ovary
Epithelium
Macrophages
Neural Cell Adhesion Molecules
Glycoproteins
Keratin-18
Granulosa Cells
Menstrual Cycle
Microvessels
Immune System
Immunohistochemistry
Phenotype

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

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title = "Immunohistochemical Studies of the Adult Human Ovary: Possible Contribution of Immune and Epithelial Factors to Folliculogenesis",
abstract = "PROBLEM: Formation of primordial follicles in adult ovaries could be a cryptic process limited to relatively small areas of the ovarian cortex and occurring during a certain stage of the menstrual cycle. Such an event may require a specific milieu provided by factors involved in developmental processes, i.e., morphoregulatory molecules and macrophages. METHOD: Adult human ovaries were investigated by immunohistochemistry for surface epithelium and granulosa cell markers (cytokeratin 18 and MHC class I), immune system‐related morphoregulatory molecules (Thy‐1 glycoprotein and N‐CAM), and macrophage phenotypes (CD14, CD68, and MHC class II). RESULTS: In some ovaries 300–500 μm areas of surface epithelium were overgrown by tunica albuginea, descended into the stroma, and apparently fragmented into individual small (20–40 μm) follicle‐like cell nests. Differentiation of the surface epithelium was accompanied by macrophages and Thy‐1 glycoprotein. Small segments of surface epithelium showed N‐CAM and a lacked MHC class I expression. In such segments, clear spherical germ‐like cells migrated into the deeper stroma, associated with the microvasculature, and eventually aggregated with follicle‐like cell nests. CONCLUSIONS: Our data suggest that surface epithelium may be involved in the formation of some primordial follicles in adult ovaries. This process, and further follicular fate, may require a precise interplay of immune system related morphoregulatory molecules and macrophages. 1995 Munksgaard",
author = "ANTON{\'I}N BuKOVSK{\'y} and Jeffrey Keenan and Michael Caudle and JAY WIMALASENA and Nirmala Upadhyaya and {Van Meter}, Stuart",
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T1 - Immunohistochemical Studies of the Adult Human Ovary

T2 - Possible Contribution of Immune and Epithelial Factors to Folliculogenesis

AU - BuKOVSKý, ANTONÍN

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AU - Caudle, Michael

AU - WIMALASENA, JAY

AU - Upadhyaya, Nirmala

AU - Van Meter, Stuart

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N2 - PROBLEM: Formation of primordial follicles in adult ovaries could be a cryptic process limited to relatively small areas of the ovarian cortex and occurring during a certain stage of the menstrual cycle. Such an event may require a specific milieu provided by factors involved in developmental processes, i.e., morphoregulatory molecules and macrophages. METHOD: Adult human ovaries were investigated by immunohistochemistry for surface epithelium and granulosa cell markers (cytokeratin 18 and MHC class I), immune system‐related morphoregulatory molecules (Thy‐1 glycoprotein and N‐CAM), and macrophage phenotypes (CD14, CD68, and MHC class II). RESULTS: In some ovaries 300–500 μm areas of surface epithelium were overgrown by tunica albuginea, descended into the stroma, and apparently fragmented into individual small (20–40 μm) follicle‐like cell nests. Differentiation of the surface epithelium was accompanied by macrophages and Thy‐1 glycoprotein. Small segments of surface epithelium showed N‐CAM and a lacked MHC class I expression. In such segments, clear spherical germ‐like cells migrated into the deeper stroma, associated with the microvasculature, and eventually aggregated with follicle‐like cell nests. CONCLUSIONS: Our data suggest that surface epithelium may be involved in the formation of some primordial follicles in adult ovaries. This process, and further follicular fate, may require a precise interplay of immune system related morphoregulatory molecules and macrophages. 1995 Munksgaard

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