Immunohistochemistry of sebaceous gland carcinoma. An analysis of 24 cases

R. L. Font, I. W. McLean, Rodolfo Laucirica

Research output: Contribution to journalArticle

Abstract

Purpose. To evaluate the immunophenotypic profile of sebaceous gland carcinoma (SGC) of the eyelid and ocular adnexa. Methods. Paraffinembedded tissues from twenty-four SGC's were evaluated using a panel of four monoclonal antibodies [epithelia. membrane antigen (EMA), gross cystic disease fluid protein-15 (GCDFP-15), HAM-56, and carcinoembryonic antigen (CEA)]. The staining pattern was subjectively scored for intensity and percent of positive cells. These re;ults were correlated with the degree of differentiation of each tumor. Results. 22 of 24 SGC's, in which tumor was available, demonstrated strong :mmunoreactivity with EMA We observed a direct correlation between tlie staining pattern with EMA and the degree of sebaceous differentiation within the tumor lobules All 24 tumors were CEA negative GCDFP-15 selectively stained the necrotic sebaceous material within the neoplastic lobules :n three tumors In 7 of 24 tumors (29%), HAM-56 predominantly showed non-specific staining of lipid-laden stromal histiocytes. The latter finding icpresents extracellular lipid from the tumor that has been phagocytized b> histiocytes. Comilusions. EMA proved to be the most reliable and con;istent immunomarker to confirm the histopathologic diagnosis of SCG.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

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Sebaceous Glands
Immunohistochemistry
Carcinoma
Neoplasms
Histiocytes
Carcinoembryonic Antigen
Staining and Labeling
Lipids
Eyelids
Proteins
Epithelium
Monoclonal Antibodies
Antigens
Membranes

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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Immunohistochemistry of sebaceous gland carcinoma. An analysis of 24 cases. / Font, R. L.; McLean, I. W.; Laucirica, Rodolfo.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 01.12.1997.

Research output: Contribution to journalArticle

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