Immunoliposome labeling

A sensitive and specific method for cell surface labeling

Anthony Huang, Stephen Kennel, Leaf Huang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A simple, one-step procedure for fluorescence labeling of cultured cells with high sensitivity and specificity is described. We term this method immunoliposome labeling. Monoclonal IgG antibody was first covalently coupled with palmitic acid. The palmitoyl-IgG was then incorporated into unilamellar liposomes (about 100 nm diameter) containing either N-(4-nitrobenzo-2-oxa-1,3-diazole)- or N-(fluorescein isothiocyanyl)-phosphatidylethanolamine by a detergent-dialysis procedure. A monoclonal antibody to the mouse major histocompatibility antigen, H-2 k , was tested as a model system. Fluorescent liposomes with covalently coupled anti-H-2 k specifically labeled the mouse L-929 cells (H-2 k type), but not the A-31 cells (H-2 d type). The degree of labeling was quantitated by a microscope photometer. Cells labeled with fluorescent liposomes showed 4-6-fold stronger fluorescence than cells labeled with either fluorescein-conjugated antibody, or with primary antibody followed by fluorescein-conjugated secondary antibody (indirect immunofluorescence). Since different types of label (fluorescent, radioactive, etc.) can be incorporated into liposomes, this specific and sensitive method is potentially very versatile.

Original languageEnglish (US)
Pages (from-to)141-151
Number of pages11
JournalJournal of Immunological Methods
Volume46
Issue number2
DOIs
StatePublished - Oct 30 1981

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Fluorescein
Liposomes
Antibodies
Immunoglobulin G
Fluorescence
Monoclonal Antibodies
Unilamellar Liposomes
Histocompatibility Antigens
Palmitic Acid
Indirect Fluorescent Antibody Technique
Detergents
Dialysis
Cultured Cells
Sensitivity and Specificity

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Immunoliposome labeling : A sensitive and specific method for cell surface labeling. / Huang, Anthony; Kennel, Stephen; Huang, Leaf.

In: Journal of Immunological Methods, Vol. 46, No. 2, 30.10.1981, p. 141-151.

Research output: Contribution to journalArticle

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