Immunological detection of high molecular weight proteins by gel and blot overlay

Beat M. Riederer, Steven Goodman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The sensitivity and specificity of the gel overlay and western blot methods of immunodetection are compared for spectrins, typical high molecular weight proteins. The gel overlay method is more sensitive and specific for the immunodetection of brain spectrin( 240 235) and rbc spectrin. As the western blot technique will remain the method of choice for many applications because of its relative speed, we discuss methods for optimizing its sensitivity and selectivity.

Original languageEnglish (US)
Pages (from-to)715-722
Number of pages8
JournalBrain Research Bulletin
Volume19
Issue number6
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

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Spectrin
Molecular Weight
Gels
Proteins
Western Blotting
Sensitivity and Specificity
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Immunological detection of high molecular weight proteins by gel and blot overlay. / Riederer, Beat M.; Goodman, Steven.

In: Brain Research Bulletin, Vol. 19, No. 6, 01.01.1987, p. 715-722.

Research output: Contribution to journalArticle

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