Immunological relationship among hydrogenases.

K. L. Kovacs, L. C. Seefeldt, Gabor Tigyi, C. M. Doyle, L. E. Mortenson, D. J. Arp

Research output: Contribution to journalArticle

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Abstract

We examined the immunological cross-reactions of 11 different hydrogenase antigens with 9 different hydrogenase antibodies. Included were antibodies and antigens of both subunits of the hydrogenases of Bradyrhizobium japonicum and Thiocapsa roseopersicina. The results showed a strong relationship among the Ni-Fe dimeric hydrogenases. The two subunits of Ni-Fe dimeric hydrogenases appeared immunologically distinct: specific interactions occurred only when antibodies to the 60- and 30-kilodalton subunits reacted with the 60- and 30-kilodalton-subunit antigens. The interspecies cross-reactions suggested that at least one conserved protein region exists among the large subunits of these enzymes, whereas the small subunits are less conserved. Antibodies to the Fe-only bidirectional hydrogenase of Clostridium pasteurianum reacted with the Desulfovibrio vulgaris bidirectional hydrogenase. Surprisingly, antibodies to the clostridial uptake hydrogenase did not react with any of the Fe-only bidirectional hydrogenases but did react with several of the Ni-Fe dimeric hydrogenases. The two hydrogenases from C. pasteurianum were found to be quite different immunologically. The possible relationship of these findings to the structure and catalytic functions of hydrogenase are discussed.

Original languageEnglish (US)
Pages (from-to)430-435
Number of pages6
JournalJournal of Bacteriology
Volume171
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

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Hydrogenase
Antibodies
Cross Reactions
Antigens
Thiocapsa roseopersicina
Desulfovibrio vulgaris
Bradyrhizobium
Clostridium
iron hydrogenase
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Kovacs, K. L., Seefeldt, L. C., Tigyi, G., Doyle, C. M., Mortenson, L. E., & Arp, D. J. (1989). Immunological relationship among hydrogenases. Journal of Bacteriology, 171(1), 430-435. https://doi.org/10.1128/jb.171.1.430-435.1989

Immunological relationship among hydrogenases. / Kovacs, K. L.; Seefeldt, L. C.; Tigyi, Gabor; Doyle, C. M.; Mortenson, L. E.; Arp, D. J.

In: Journal of Bacteriology, Vol. 171, No. 1, 01.01.1989, p. 430-435.

Research output: Contribution to journalArticle

Kovacs, KL, Seefeldt, LC, Tigyi, G, Doyle, CM, Mortenson, LE & Arp, DJ 1989, 'Immunological relationship among hydrogenases.', Journal of Bacteriology, vol. 171, no. 1, pp. 430-435. https://doi.org/10.1128/jb.171.1.430-435.1989
Kovacs KL, Seefeldt LC, Tigyi G, Doyle CM, Mortenson LE, Arp DJ. Immunological relationship among hydrogenases. Journal of Bacteriology. 1989 Jan 1;171(1):430-435. https://doi.org/10.1128/jb.171.1.430-435.1989
Kovacs, K. L. ; Seefeldt, L. C. ; Tigyi, Gabor ; Doyle, C. M. ; Mortenson, L. E. ; Arp, D. J. / Immunological relationship among hydrogenases. In: Journal of Bacteriology. 1989 ; Vol. 171, No. 1. pp. 430-435.
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