Immunological response to (1,4)-α-d-glucan in the lung and spleen of endotoxin-stimulated juvenile rats

Ectis A. Velazquez, Dai Kimura, Dan Torbati, Cheppail Ramachandran, Balagangadhar R. Totapally

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of (1,4)-α-D-glucan (α-DG), a novel immune stimulatory drug from Tinospora cordifolia, on the concentration of pro- and anti-inflammatory cytokines (interleukin [IL]-1β, IL-6, tumour necrosis factor-α [TNF-α], γ-interferon [IFN-γ] and IL-10) in the lung and spleen of endotoxin-stimulated juvenile rats. Experimental groups (n = 16/group) included controls with an intraperitoneal injection of saline, endotoxaemic rats with a non-lethal dose of 10 mg/kg Escherichia coli endotoxin, and endotoxaemic rats treated with two doses of 10 mg/kg α-DG, intraperitoneally, 2 and 4 hr after endotoxin injection. At 24 hr of treatment, rats were euthanized and lungs and spleen were removed for cytokines determination and lung injury. Endotoxaemia increased IL-1β concentration by fivefold in both organs, while creating a moderate pulmonary hypercellularity (demonstrated by about 11% increase in the alveolar-septal thickening and 11% decrease in the alveolar-interstitial space ratio). In the lung, α-DG treatment reduced concentrations of IL-1β by 30% (p > 0.05), IL-6 by 43% (p < 0.01), IFN-γ by 46% (p < 0.01) and the anti-inflammatory cytokine, IL-10, by 31% (p > 0.05) compared to endotoxaemia. In the spleen, α-DG treatment decreased the ratio of IL-1β to IL-10 by 55% (p < 0.05), demonstrating an anti-inflammatory trend. These data suggest that α-DG differentially modulates cytokine response in the lung and spleen and modifies the pro- and anti-inflammatory balance during an early period of endotoxaemia in juvenile rats.

Original languageEnglish (US)
Pages (from-to)301-306
Number of pages6
JournalBasic and Clinical Pharmacology and Toxicology
Volume105
Issue number5
DOIs
StatePublished - Nov 1 2009

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Glucans
Endotoxins
Rats
Interleukin-1
Spleen
Endotoxemia
Lung
Anti-Inflammatory Agents
Cytokines
Interleukin-10
Interleukin-6
Tinospora
Lung Injury
Intraperitoneal Injections
Interferons
Therapeutics
Tumor Necrosis Factor-alpha
1,4-glucan
Control Groups
Injections

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Pharmacology

Cite this

Immunological response to (1,4)-α-d-glucan in the lung and spleen of endotoxin-stimulated juvenile rats. / Velazquez, Ectis A.; Kimura, Dai; Torbati, Dan; Ramachandran, Cheppail; Totapally, Balagangadhar R.

In: Basic and Clinical Pharmacology and Toxicology, Vol. 105, No. 5, 01.11.2009, p. 301-306.

Research output: Contribution to journalArticle

Velazquez, Ectis A. ; Kimura, Dai ; Torbati, Dan ; Ramachandran, Cheppail ; Totapally, Balagangadhar R. / Immunological response to (1,4)-α-d-glucan in the lung and spleen of endotoxin-stimulated juvenile rats. In: Basic and Clinical Pharmacology and Toxicology. 2009 ; Vol. 105, No. 5. pp. 301-306.
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