Immunomodulation by exogenous surfactant

Effect on TNF-α secretion and luminol-enhanced chemiluminescence activity by murine macrophages stimulated with group B streptococci

Ajay Talati, Dennis T. Crouse, B. Keith English, Cynthia Newman, Lisa Harrison, Elizabeth Meals

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Group B streptococci (GBS) are important pathogens in neonatal sepsis and pneumonia. GBS stimulate alveolar macrophages to produce inflammatory cytokines and free oxygen radicals, which can damage the lungs. In several studies, use of exogenous surfactant in term babies has improved outcome related to sepsis and respiratory failure. The role(s) of exogenous surfactant in modulating the inflammatory response produced by this microbe was examined. Tumor necrosis factor alpha (TNF-α) production and luminol-enhanced chemiluminescence (LCL), a measure of respiratory burst, were investigated. For measuring TNF-α release, RAW 264.7 murine macrophages were pre-incubated with bovine surfactant and stimulated with either lipopolysaccharide, live or heat-killed GBS type Ia. LCL was measured after macrophages were pre-incubated with or without surfactant overnight, then stimulated with GBS or phorbol myristate acetate. Lipopolysaccharide and GBS stimulated TNF-α secretion from macrophages that was suppressed by exogenous surfactant in a dose-dependent fashion. GBS and phorbol myristate acetate also increased LCL from macrophages, which was significantly suppressed by pre-incubation of macrophages with exogenous surfactant. We conclude that GBS type Ia stimulates TNF-α release and LCL from RAW 264.7 cells and that these responses are suppressed by surfactant. Suppression of inflammatory mediators by exogenous surfactant might improve respiratory disease associated with GBS.

Original languageEnglish (US)
Pages (from-to)267-273
Number of pages7
JournalMicrobes and Infection
Volume3
Issue number4
DOIs
StatePublished - Jan 1 2001

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Luminol
Streptococcus agalactiae
Immunomodulation
Luminescence
Surface-Active Agents
Tumor Necrosis Factor-alpha
Macrophages
Tetradecanoylphorbol Acetate
Respiratory Burst
Alveolar Macrophages
Streptococcus
Respiratory Insufficiency
Free Radicals
Lipopolysaccharides
Reactive Oxygen Species
Sepsis
Pneumonia
Hot Temperature
Cytokines
Lung

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Immunomodulation by exogenous surfactant : Effect on TNF-α secretion and luminol-enhanced chemiluminescence activity by murine macrophages stimulated with group B streptococci. / Talati, Ajay; Crouse, Dennis T.; Keith English, B.; Newman, Cynthia; Harrison, Lisa; Meals, Elizabeth.

In: Microbes and Infection, Vol. 3, No. 4, 01.01.2001, p. 267-273.

Research output: Contribution to journalArticle

Talati, Ajay ; Crouse, Dennis T. ; Keith English, B. ; Newman, Cynthia ; Harrison, Lisa ; Meals, Elizabeth. / Immunomodulation by exogenous surfactant : Effect on TNF-α secretion and luminol-enhanced chemiluminescence activity by murine macrophages stimulated with group B streptococci. In: Microbes and Infection. 2001 ; Vol. 3, No. 4. pp. 267-273.
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